Assessing Temperature Responses in Roots

Methods Mol Biol. 2024:2795:37-42. doi: 10.1007/978-1-0716-3814-9_4.

Abstract

Due to global warming, it is important to understand how plants respond to high ambient temperature. Plant growth responses to high ambient temperature are termed thermomophogenesis and have been explored for more than a decade. However, this was mostly focused on the above-ground part of plants, the shoot. In this chapter, we describe a simple method to assess root growth phenotype to high ambient temperatures. In principle, this protocol can be applied for any other treatments to test overall seedling growth.

Keywords: Arabidopsis thaliana; Root; Root phenotyping; Thermomorphogenesis.

MeSH terms

  • Arabidopsis* / genetics
  • Plant Roots / genetics
  • Seedlings
  • Temperature