Carotenoids and Tocopherol Profiling in Fleshy Fruits

Bruno Silvestre Lira; Aline Bertinatto Cruz; Magdalena Rossi; Luciano Freschi

doi:10.1007/978-1-0716-3826-2_9

Carotenoids and Tocopherol Profiling in Fleshy Fruits

Methods Mol Biol. 2024:2798:141-151. doi: 10.1007/978-1-0716-3826-2_9.

Authors

Bruno Silvestre Lira¹, Aline Bertinatto Cruz¹, Magdalena Rossi¹, Luciano Freschi²

Affiliations

¹ Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil.
² Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil. freschi@usp.br.

PMID: 38587740
DOI: 10.1007/978-1-0716-3826-2_9

Abstract

Carotenoids and tocopherols are among the most powerful lipophilic antioxidants accumulated in fruit and vegetable crops. This chapter describes a method for the separation and quantification of carotenoids/chlorophylls and tocopherols based on microextraction followed by reverse- and normal-phase HPLC, respectively. Using this method, high-throughput, accurate analysis of these compounds can be performed in leaf and fruit samples.

Keywords: Antioxidants; Carotenoids; Flesh fruits; Lycopene; Ripening; Tocopherols; Tomato.

MeSH terms

Antioxidants
Carotenoids*
Fruit
Tocopherols*
Vitamin E

Substances

Tocopherols
Carotenoids
Vitamin E
Antioxidants