SS-31 inhibits the inflammatory response by increasing ATG5 and promoting autophagy in lipopolysaccharide-stimulated HepG2 cells

Yunan Mo; Songyun Deng; Yuhang Ai; Wenchao Li

doi:10.1016/j.bbrc.2024.149887

SS-31 inhibits the inflammatory response by increasing ATG5 and promoting autophagy in lipopolysaccharide-stimulated HepG2 cells

Biochem Biophys Res Commun. 2024 May 28:710:149887. doi: 10.1016/j.bbrc.2024.149887. Epub 2024 Apr 4.

Authors

Yunan Mo¹, Songyun Deng², Yuhang Ai³, Wenchao Li⁴

Affiliations

¹ Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China; Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China. Electronic address: moyunan@csu.edu.cn.
² Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China; Department of Plastic Surgery, Yaoyanzhi Aesthetic Hospital, Haikou, Hainan, 570203, China. Electronic address: dengsy2014@foxmail.com.
³ Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China. Electronic address: ayhicu1978@csu.edu.cn.
⁴ Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China; Emergency Department of Internal Medicine, Emergency Trauma Center, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 830000, China. Electronic address: 178101067@csu.edu.cn.

PMID: 38581954
DOI: 10.1016/j.bbrc.2024.149887

Abstract

SS-31 is a mitochondria-targeting short peptide. Recent studies have indicated its hepatoprotective effects. In our study, we investigated the impact of SS-31 on LPS-induced autophagy in HepG2 cells. The results obtained from a dual-fluorescence autophagy detection system revealed that SS-31 promotes the formation of autolysosomes and autophagosomes, thereby facilitating autophagic flux to a certain degree. Additionally, both ELISA and qPCR analyses provided further evidence that SS-31 safeguards HepG2 cells against inflammatory responses triggered by LPS through ATG5-dependent autophagy. In summary, our study demonstrates that SS-31 inhibits LPS-stimulated inflammation in HepG2 cells by upregulating ATG5-dependent autophagy.

Keywords: ATG5; Autophagosomes; Autophagy; Lipopolysaccharide; SS-31.

MeSH terms

Autophagosomes
Autophagy*
Autophagy-Related Protein 5 / genetics
Hep G2 Cells
Humans
Inflammation
Lipopolysaccharides* / pharmacology

Substances

Lipopolysaccharides
ATG5 protein, human
Autophagy-Related Protein 5