Changes in inoculated Salmonella enterica subsp. enterica Serovar Enteritidis and other microbiological qualities of vacuum-packed carrot slices after treatment with aqueous extract of Lobularia maritima

Anis Ben Hsouna; Natália Čmiková; Boutheina Ben Akacha; Rania Ben Saad; Wissem Mnif; Stefania Garzoli; Miroslava Kačániová

doi:10.1016/j.heliyon.2024.e29065

Changes in inoculated Salmonella enterica subsp. enterica Serovar Enteritidis and other microbiological qualities of vacuum-packed carrot slices after treatment with aqueous extract of Lobularia maritima

Heliyon. 2024 Mar 30;10(7):e29065. doi: 10.1016/j.heliyon.2024.e29065. eCollection 2024 Apr 15.

Authors

Anis Ben Hsouna^{1

2}, Natália Čmiková³, Boutheina Ben Akacha¹, Rania Ben Saad¹, Wissem Mnif⁴, Stefania Garzoli⁵, Miroslava Kačániová^{3

6}

Affiliations

¹ Laboratory of Biotechnology and Plant Improvement, Centre of Biotechnology of Sfax, P.O. Box 1177, Sfax, 3018, Tunisia.
² Department of Environmental Sciences and Nutrition, Higher Institute of Applied Sciences and Technology of Mahdia, University of Monastir, Monastir, 5000, Tunisia.
³ Institute of Horticulture, Faculty of Horticulture and Landscape Engineering, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76, Nitra, Slovak Republic.
⁴ Department of Chemistry, College of Sciences at Bisha, University of Bisha, P.O. Box 199, Bisha, 61922, Saudi Arabia.
⁵ Department of Chemistry and Technologies of Drug, Sapienza University, P. le Aldo Moro, 5, 00185, Rome, Italy.
⁶ School of Medical & Health Sciences, University of Economics and Human Sciences in Warsaw, Okopowa 59, Warszawa, 01 043, Poland.

Abstract

After harvesting, pathogens can infect fresh vegetables in different ways. Pathogenic bacteria associated with fresh vegetables can cause widespread epidemics associated with foodborne illness. The aim of this study was to assess the microbiological quality of carrot slices after treatment with aqueous extracts of Lobularia maritima (AELm) at different concentrations AELm1 (10 mg/mL), AELm2 (5 mg/mL), AELm3 (2.5 mg/mL) and AELm4 (1.25 mg/mL), and Salmonella enterica subsp. enterica serovar Enteritidis, along with vacuum packaging and storage of carrots for 7 days at 4 °C. On days 1. and 7., total viable counts (TVC), and coliforms bacteria (CB), and Salmonella count were all analysed. Microorganisms that were obtained from carrots were identified using MALDI-TOF MS Biotyper Mass Spectrometry. The total viable, coliform bacteria and Salmonella counts were varied by the group of treatment. Higher counts were found in the control group on both days. The most isolated species of bacteria were Salmonella enterica and Pantoea agglomerans on the 1. day and Klebsiella oxytoca on the 7. day. The current study adds useful information for a better understanding of how Salmonella enterica reacts to the effect of AELm and its potential use as a sustainable washing method to eliminate bacteria from freshly cut carrots.

Keywords: Antimicrobial activity; Aqueous extract; Carrot; Lobularia maritima; Salmonella enterica.