The study was designed to evaluate the serum alkaline phosphatase level may be altered in postmenopausal women. This case-control study was carried out in the Department of the Biochemistry, Mymensingh Medical College, Bangladesh from January 2015 to December 2015. The subjects were selected on the basis of inclusion and exclusion criteria by purposive (non-random) method. This study included 50 postmenopausal women as case. The results were compared with 50 apparently healthy pre-menopausal women as control. All statistical analysis was done by SPSS windows package. The values were expressed as Mean±SD. Statistical significance of difference between two groups was evaluated by using student's unpaired t-test. Serum alkaline phosphatase level was analyzed. Serum alkaline phosphatase was determined by using colorimetric method. The mean value of serum alkaline phosphatase was 325.56±76.79 U/L respectively in Group B (Case) and 136.50±76.50 U/L respectively in Group A (Control). The level of serum alkaline phosphatase was significantly increased in Group B (Case). Menopause has an effect on serum alkaline phosphatase which leads to increased risk of development of osteoporosis. This study may facilated the clinicians and gynaecologists to update their knowledge in regard to serum alkaline phosphatase level of women associated with menopause.