Mass Spectrometric De Novo Sequencing of Natural Peptides

Abstract

Natural peptides secreted under stress conditions by many organisms are bioactive molecules with a broad spectrum of activities. These molecules could become potential models for novel pharmaceuticals, to which bacteria, according to modern scientific concepts, do not have and cannot develop resistance. Taking this into consideration, it is necessary to clarify the amino acid sequences of such peptides. Here we describe our approach to de novo sequencing of amphibians' skin secretion peptides.

Keywords: Bioactive peptides; CID; EThcD; HCD; Manual de novo sequencing; Mass spectrometry; Natural peptides.