PdRabG3f interfered with gibberellin-mediated internode elongation and xylem developing in poplar

Jiujun Du; Hantian Wei; Xueqin Song; Lei Zhang; Jianjun Hu

doi:10.1016/j.plantsci.2024.112074

PdRabG3f interfered with gibberellin-mediated internode elongation and xylem developing in poplar

Plant Sci. 2024 Jun:343:112074. doi: 10.1016/j.plantsci.2024.112074. Epub 2024 Mar 26.

Authors

Jiujun Du¹, Hantian Wei¹, Xueqin Song², Lei Zhang³, Jianjun Hu⁴

Affiliations

¹ State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
² State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu 210037, China.
³ State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu 210037, China. Electronic address: leizhang1142@caf.ac.cn.
⁴ State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu 210037, China. Electronic address: hujj@caf.ac.cn.

PMID: 38548138
DOI: 10.1016/j.plantsci.2024.112074

Abstract

As a member of the small GTPases family, Rab GTPases play a key role in specifying transport pathways in the intracellular membrane trafficking system and are involved in plant growth and development. By quantitative trait locus (QTL) mapping, PdRabG3f was identified as a candidate gene associated with shoot height in a hybrid offspring of Populus deltoides 'Danhong' × Populus simonii 'Tongliao1'. PdRabG3f localized to the nucleus, endoplasmic reticulum and tonoplast and was primarily expressed in the xylem and cambium. Overexpression of PdRabG3f in Populus alba × Populus glandulosa (84 K poplar) had inhibitory effects on vertical and radical growth. In the transgenic lines, there were evident changes in the levels of 15 gibberellin (GA) derivatives, and the application of exogenous GA₃ partially restored the phenotypes mediated by GAs deficiency. The interaction between PdRabG3f and RIC4, which was the GA-responsive factor, provided additional explanation for PdRabG3f's inhibitory effect on poplar growth. RNA-seq analysis revealed differentially expressed genes (DEGs) associated with cell wall, xylem, and gibberellin response. PdRabG3f interfering endogenous GAs levels in poplar might involve the participation of MYBs and ultimately affected internode elongation and xylem development. This study provides a potential mechanism for gibberellin-mediated regulation of plant growth through Rab GTPases.

Keywords: Cell wall; Gibberellin; Internode elongation; Populus; QTL; Xylem.

MeSH terms

Gene Expression Regulation, Plant
Gibberellins* / metabolism
Plants, Genetically Modified / genetics
Populus* / metabolism
Xylem
rab GTP-Binding Proteins / genetics
rab GTP-Binding Proteins / metabolism

Substances

Gibberellins
rab GTP-Binding Proteins