One Step Forwards in Knowledge of Blossom Blight Brown Rot Disease: Monilinia spp. SSR Marker Database

Raminta Antanynienė; Vidmantas Stanys; Birutė Frercks

doi:10.3390/microorganisms12030605

One Step Forwards in Knowledge of Blossom Blight Brown Rot Disease: Monilinia spp. SSR Marker Database

Microorganisms. 2024 Mar 18;12(3):605. doi: 10.3390/microorganisms12030605.

Authors

Raminta Antanynienė¹, Vidmantas Stanys¹, Birutė Frercks¹

Affiliation

¹ Lithuanian Research Centre for Agriculture and Forestry, Institute of Horticulture, Department of Orchard Plant Genetics and Biotechnology, Kaunas District, LT-54333 Babtai, Lithuania.

Abstract

A freely available Monilinia spp. marker database was created, containing microsatellite (SSR) data of the three most essential European fungal pathogens: M. fructigena, M. laxa, and M. fructicola. These pathogens cause brown rot blossom blight. Microsatellites were identified using the bioinformatics tool Genome-wide Microsatellite Analyzing Toward Application (GMATA). The database provides information about SSR markers: forward and reverse sequences of the primers, fragment sizes, SSR motifs (and repeats), and the exact locations with the coordinates in the reference genome. This database currently contains information about 39,216 SSR motifs and 26,366 markers. In total, eight primers generated in silico were validated experimentally and they are marked in the database. All scientists can join this collaboration by adding their experimental data. This database is the initial start of organizing Monilinia spp. molecular data worldwide and, in the future, it could be extended by adding more molecular and genomic information.

Keywords: Monilinia spp.; database; microsatellite; molecular markers.

Grants and funding

The study was supported by the long-term research program “Advances in genetics, biotechnology and breeding for improved plant diversity and technological innovations” implemented by LRCAF.