Enhanced Heat Resistance in Morchella eximia by Atmospheric and Room Temperature Plasma

Qin Zhang; Junbin Lin; Junjie Yan; Renyun Miao; Rencai Feng; Ying Gan; Bingcheng Gan

doi:10.3390/microorganisms12030518

Enhanced Heat Resistance in Morchella eximia by Atmospheric and Room Temperature Plasma

Microorganisms. 2024 Mar 5;12(3):518. doi: 10.3390/microorganisms12030518.

Authors

Qin Zhang^{1

2}, Junbin Lin^{1

2}, Junjie Yan^{1

2}, Renyun Miao^{1

2}, Rencai Feng^{1

2}, Ying Gan^{1

2}, Bingcheng Gan^{1

2}

Affiliations

¹ Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610299, China.
² Chengdu National Agricultural Science and Technology Center, Chengdu 610299, China.

Abstract

This study focuses on optimizing the mutagenesis process for Morchella eximia (Mel-7) mycelia through atmospheric and room temperature plasma (ARTP) mutation and explores the resultant thermal adaptability and physiological responses of mutant strains. This research demonstrated a clear relationship between ARTP mutagenesis exposure duration and lethality rate, indicating that an exposure time of 40 s resulted in the optimal balance of inducing mutations without causing excessive mortality. Additionally, this study established 43 °C as the ideal screening temperature for identifying mutant strains with enhanced heat resistance, as this temperature significantly challenges the mycelia while allowing thermotolerant strains to be distinguishable. Among the screened mutants, strains L21, L23, L44, and L47 exhibited superior growth and high-temperature tolerance, with notable resilience at 30 °C, highlighting their enhanced adaptability to above-optimal temperatures. Furthermore, this research delved into biochemical responses, including lipid peroxidation and non-enzymatic antioxidant content, highlighting the diverse mechanisms, such as enhanced lipid peroxidation resistance and increased antioxidant content, employed by mutant strains to adapt to temperature fluctuations. The activities of antioxidant enzymes, including peroxidase (POD) and superoxide dismutase (SOD), were shown to be significantly influenced by temperature elevations, illustrating their critical roles in the thermal adaptation of mutant strains. These findings shed light on the importance of considering mutation duration and temperature screening in the development of thermotolerant fungal strains with potential applications in various industries. This study's breakthrough lies in its comprehensive understanding of the thermal adaptability of Mel-7 mycelia and the identification of promising mutant strains, offering valuable insights for both academic and industrial purposes.

Keywords: ARTP mutagenesis; Mel-7; antioxidant enzyme activity; glutathione; heat resistance.

Abstract

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