PIV-MyoMonitor: an accessible particle image velocimetry-based software tool for advanced contractility assessment of cardiac organoids

Hoyeon Lee; Boyoung Kim; Jiyue Yun; Jinseung Bae; Sungsu Park; Junseok Jeon; Hye Ryoun Jang; Jaecheol Lee; Soah Lee

doi:10.3389/fbioe.2024.1367141

PIV-MyoMonitor: an accessible particle image velocimetry-based software tool for advanced contractility assessment of cardiac organoids

Front Bioeng Biotechnol. 2024 Mar 12:12:1367141. doi: 10.3389/fbioe.2024.1367141. eCollection 2024.

Authors

Hoyeon Lee^{1

2}, Boyoung Kim¹, Jiyue Yun¹, Jinseung Bae³, Sungsu Park³, Junseok Jeon⁴, Hye Ryoun Jang⁴, Jaecheol Lee^{1

5

6}, Soah Lee^{1

5}

Affiliations

¹ Department of Biopharmaceutical Convergence, Sungkyunkwan University, Suwon, Republic of Korea.
² School of Electronic and Electrical Engineering, Sungkyunkwan University, Suwon, Republic of Korea.
³ School of Mechanical Engineering, Sungkyunkwan University, Suwon, Republic of Korea.
⁴ Division of Nephrology, Department of Medicine, Cell and Gene Therapy Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
⁵ School of Pharmacy, Sungkyunkwan University, Suwon, Republic of Korea.
⁶ Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon, Republic of Korea.

Abstract

Induced pluripotent stem cell (iPSC)-derived cardiac organoids offer a versatile platform for personalized cardiac toxicity assessment, drug screening, disease modeling, and regenerative therapies. While previous image-based contractility analysis techniques allowed the assessment of contractility of two-dimensional cardiac models, they face limitations, including encountering high noise levels when applied to three-dimensional organoid models and requiring expensive equipment. Additionally, they offer fewer functional parameters compared to commercial software. To address these challenges, we developed an open-source, particle image velocimetry-based software (PIV-MyoMonitor) and demonstrated its capacity for accurate contractility analysis in both two- and three-dimensional cardiac models using standard lab equipment. Comparisons with four other open-source software programs highlighted the capability of PIV-MyoMonitor for more comprehensive quantitative analysis, providing 22 functional parameters and enhanced video outputs. We showcased its applicability in drug screening by characterizing the response of cardiac organoids to a known isotropic drug, isoprenaline. In sum, PIV-MyoMonitor enables reliable contractility assessment across various cardiac models without costly equipment or software. We believe this software will benefit a broader scientific community.

Keywords: IPSC-CM cardiomyocytes; cardiac models; contractility; induced pluripotent stem cells; organoid; particle image velocimetry.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education (2022R1A6A1A03054419), Korean Fund for Regenerative Medicine funded by Ministry of Science and ICT, and Ministry of Health and Welfare (22A0302L1-01, Republic of Korea), a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic of Korea (grant number: HR22C1363), and the National Research Foundation of Korea(NRF) grant funded by the Korea government(MSIT) (RS-2023-00250723).