Measurement of the Kinetics of Chlorophyll a Fluorescence by an LED-Light Source Fluorimeter, Handy PEA

Methods Mol Biol. 2024:2791:121-126. doi: 10.1007/978-1-0716-3794-4_13.

Abstract

The chlorophyll a fluorescence measurement method is used to determine the efficiency of the photosynthetic apparatus and to assess the physiological state of photosynthetic organisms. The measurement is simple, fast, and noninvasive. It is a precise tool to study photosynthesis response under stress conditions or to assess the impact of specific environmental factors on plants. Here we describe the usage of this method in environmental-controlled plant production systems differing in temperature or light source on the growth and development of common buckwheat.

Keywords: Chlorophyll fluorescence; Noninvasive method; Photosynthesis; Photosystem II; Stress factor.

MeSH terms

  • Chlorophyll A
  • Chlorophyll*
  • Fluorescence
  • Kinetics
  • Photosynthesis / physiology
  • Photosystem II Protein Complex / metabolism
  • Pisum sativum*
  • Plant Leaves / metabolism

Substances

  • Chlorophyll A
  • Chlorophyll
  • Photosystem II Protein Complex