Two-Dimensional Gel Electrophoresis in Studies of Flower and Leaf Proteome of Common Buckwheat

Przemysław Kopeć; Monika Krzewska; Agnieszka Płażek

doi:10.1007/978-1-0716-3794-4_12

Two-Dimensional Gel Electrophoresis in Studies of Flower and Leaf Proteome of Common Buckwheat

Methods Mol Biol. 2024:2791:113-119. doi: 10.1007/978-1-0716-3794-4_12.

Authors

Przemysław Kopeć¹, Monika Krzewska², Agnieszka Płażek³

Affiliations

¹ The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków, Poland. p.kopec@ifr-pan.edu.pl.
² The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków, Poland.
³ Department of Physiology, Breeding of Plant and Seed Science, University of Agriculture, Cracow, Poland.

PMID: 38532098
DOI: 10.1007/978-1-0716-3794-4_12

Abstract

Two-dimensional gel electrophoresis (2-DE) is a proteomic tool used for the separation of protein mixtures according to protein isoelectric point and molecular mass. Although gel-free quantitative and qualitative proteomic study techniques are now available, 2-DE remains a useful analytical tool. The presented protocol was performed to analyze the flower and leaf proteome of common buckwheat using 24 cm immobilized pH gradient strips (pH 4-7) and visualization of proteins on gels via colloidal Coomassie G-250 staining.

Keywords: Colloidal Coomassie staining; Isoelectric focusing; Proteome analysis; SDS-PAGE; Two-dimensional gel electrophoresis.

MeSH terms

Electrophoresis, Gel, Two-Dimensional / methods
Fagopyrum*
Flowers
Gels
Hydrogen-Ion Concentration
Isoelectric Focusing / methods
Plant Leaves / chemistry
Proteome* / analysis
Proteomics

Substances

Proteome
Gels