An overview of aptamer: Design strategy, prominent applications, and potential challenge in plants

Yanhui Yuan; Yi Li; Siying Liu; Pichang Gong; Jinxing Lin; Xi Zhang

doi:10.1016/j.jplph.2024.154235

An overview of aptamer: Design strategy, prominent applications, and potential challenge in plants

J Plant Physiol. 2024 May:296:154235. doi: 10.1016/j.jplph.2024.154235. Epub 2024 Mar 19.

Authors

Yanhui Yuan¹, Yi Li¹, Siying Liu², Pichang Gong³, Jinxing Lin¹, Xi Zhang⁴

Affiliations

¹ State Key Laboratory of Tree Genetics and Breeding, State Key Laboratory of Efficient Production of Forest Resources, National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China; Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing, 100083, China.
² State Key Laboratory of Tree Genetics and Breeding, State Key Laboratory of Efficient Production of Forest Resources, National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
³ State Key Laboratory of Plant Diversity and Specialty Crops, Institute of Botany, the Chinese Academy of Sciences, Beijing, 100093, China.
⁴ State Key Laboratory of Tree Genetics and Breeding, State Key Laboratory of Efficient Production of Forest Resources, National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China; Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing, 100083, China. Electronic address: zhangxi@bjfu.edu.cn.

PMID: 38531181
DOI: 10.1016/j.jplph.2024.154235

Abstract

Aptamers, serving as highly efficient molecular recognition and biotechnology tools, have garnered increasing interest in the realm of plant science in recent years. Aptamers are synthetic single-stranded short nucleotides or peptides, that bind targets with high specificity and affinity, triggering precise biological responses. As an alternative to antibodies, aptamers present promising avenues for advancement in biological researches. Aptamers function in a range of fields, encompassing cell signaling, drug development, biosensor technology, as well as botany, agricultural and forestry sciences. In this review, we introduce classifications and screening methods of aptamers, as well as aptamer-based technologies, highlighting their significant contributions to recent advancements. With their powerful functionality and ability to bind targets with high specificity and affinity, aptamers offer promising opportunities for breakthroughs in plant research.

Keywords: Applications; Nucleic acid aptamer; Peptide aptamer; Plants; Screening technology.

Publication types

Review

MeSH terms

Aptamers, Nucleotide*
Biosensing Techniques*
Biotechnology

Substances

Aptamers, Nucleotide