Pharmacokinetics and relative bioavailability study of two cefquinome sulfate intramammary infusions in cow milk

Shuang Li; Na Yu; Yaoxin Tang; Chunshuang Liu; Ying Zhang; Xiaojie Chen; Hao Wu; Xiubo Li; Yiming Liu

doi:10.3389/fvets.2024.1384076

Pharmacokinetics and relative bioavailability study of two cefquinome sulfate intramammary infusions in cow milk

Front Vet Sci. 2024 Mar 11:11:1384076. doi: 10.3389/fvets.2024.1384076. eCollection 2024.

Authors

Shuang Li^#^{1

2

3}, Na Yu^#^{1

3

4}, Yaoxin Tang^{1

3

4}, Chunshuang Liu^{1

3

4}, Ying Zhang^{1

3

4}, Xiaojie Chen^{1

3

4}, Hao Wu⁵, Xiubo Li^{1

3

4}, Yiming Liu^{1

3

4}

Affiliations

¹ National Feed Drug Reference Laboratories, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
² Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, China.
³ Key Laboratory of Animal Antimicrobial Resistance Surveillance, Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
⁴ Laboratory of Quality and Safety Risk Assessment for Products on Feed-origin Risk Factor, Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
⁵ College of Veterinary Medicine, China Agricultural University, Beijing, China.

^# Contributed equally.

Abstract

In this study, two intramammary infusions of cefquinome sulfate were investigated for pharmacokinetics and bioavailability. Twelve lactating cows for each group were administered an effective dose of 75 mg/gland for cefquinome, with milk samples collected at various time intervals. The concentrations of cefquinome in milk at different times were determined by the UPLC-MS/MS method. Analyses of noncompartmental pharmacokinetics were conducted on the concentration of cefquinome in milk. Mean pharmacokinetic parameters of group A and group B following intramammary administration were as follows: AUC_last 300558.57 ± 25052.78 ng/mL and 266551.3 ± 50654.85 ng/mL, C_max 51786.35 ± 11948.4 ng/mL and 59763.7 ± 8403.2 ng/mL, T_1/2 5.69 ± 0.62 h and 5.25 ± 1.62 h, MRT 7.43 ± 0.79 h and 4.8 ± 0.78 h, respectively. Pharmacokinetic experiments showed that the relative bioavailability of group B was 88.69% that of group A. From our findings, group B (3 g: 75 mg) shows a quicker drug elimination process than group A (8 g: 75 mg), which suggests that the withdrawal period for the new formulation may be shorter.

Keywords: UPLC-MS/MS; cefquinome sulfate intramammary infusion; cow milk; pharmacokinetics; relative bioavailability.

Grants and funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This work was funded by National Key Research and Development Program of China (grant no. 2023YFD1800100) and National Key Research and Development Project (2021YFD1800700).