Synthesis, characterization of poly L(+) lactic acid and its application in sustained release of isosorbide dinitrate

El-Refaie Kenawy; A M Abd El Hay; Nermeen Saad; Mohamed M Azaam; Kamel R Shoueir

doi:10.1038/s41598-024-56222-y

Synthesis, characterization of poly L(+) lactic acid and its application in sustained release of isosorbide dinitrate

Sci Rep. 2024 Mar 25;14(1):7062. doi: 10.1038/s41598-024-56222-y.

Authors

El-Refaie Kenawy¹, A M Abd El Hay^{2

3}, Nermeen Saad⁴, Mohamed M Azaam⁴, Kamel R Shoueir⁵

Affiliations

¹ Polymer Research Group, Department of Chemistry, Faculty of Science, Tanta University, Tanta, 31527, Egypt. ekenawy@science.tanta.edu.eg.
² Chemistry Department, Faculty of Science, Al Azhar University, Cairo, Egypt.
³ Regional Technical Manager, Pharma Business Group, IMCD, Cairo, Egypt.
⁴ Polymer Research Group, Department of Chemistry, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
⁵ Institute of Nanoscience and Nanotechnology, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt. kamel_rezk@nano.kfs.edu.eg.

Abstract

Poly L(+) lactic acid (PLLA) has become crucial in the biomedical industry for various uses. The direct polycondensation method was used to prepare Poly L(+) Lactic Acid (PLLA). Different catalysts, including metal oxides and metal halides, were used to test the polymerization technique. The effect of the amount of catalysts and the type of coupling agent were investigated. The effect of reaction time and polymerization solvents was also studied. PLLA was loaded with isosorbide dinitrate utilizing the solvent evaporation process. The synthesized polymer-drug system was evaluated by different means such as FT-IR, TGA, DSC, XRD, entrapment efficiency (E.E), drug loading (D.L), particle size analysis, and zeta potential determination. Studies on in-vitro release using UV light at 227 nm at various pH levels were conducted, and the kinetics of release and cytotoxicity using the sulforhodamine B (SRB) assay on human skin fibroblast cells were examined.

Keywords: Isosorbide dinitrate; Kinetics; Nanoencapsulation; Poly l(+) lactic acid; Polycondensation polymerization; Sustained release.