Practicalities of Cell Sorting

Mark Cheetham; Derek Davies; Christopher Hall; Charlotte Christie Petersen; Reiner Schulte; Rachael Walker

doi:10.1007/978-1-0716-3738-8_7

Practicalities of Cell Sorting

Methods Mol Biol. 2024:2779:125-143. doi: 10.1007/978-1-0716-3738-8_7.

Authors

Mark Cheetham¹, Derek Davies², Christopher Hall³, Charlotte Christie Petersen⁴, Reiner Schulte⁵, Rachael Walker⁶

Affiliations

¹ , Cambridge, UK.
² Francis Crick Institute, London, UK.
³ Flow Cytometry Facility, Babraham Institute, Cambridge, UK.
⁴ The FACS Core Facility, Aarhus University, Aarhus, Denmark.
⁵ Flow Cytometry Facility, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK.
⁶ Flow Cytometry Facility, Babraham Institute, Cambridge, UK. rachael.walker@babraham.ac.uk.

PMID: 38526785
DOI: 10.1007/978-1-0716-3738-8_7

Abstract

Cell sorting is a technique commonly used in academic and biotechnology laboratories in order to separate out cells or particles of interest from heterogeneous populations. Cell sorters use the same principles as flow cytometry analyzers, but instead of cell populations passing to the waste of the instrument, they can be collected for further studies including DNA sequencing as well as other genomic, in vitro and in vivo experiments. This chapter aims to give an overview of cell sorting, the different types of cell sorters, details on how a cell sorter works, as well as protocols that are useful when embarking on a journey with cell sorting.

Keywords: Cell sorting; Flow cytometry; Fluorescence-activated cell sorting (FACS).

MeSH terms

Cell Separation / methods
Flow Cytometry / methods
Laboratories*