Isolation of Bacteriophages on Actinobacteria Hosts

Michelle Zorawik; Deborah Jacobs-Sera; Amanda C Freise; SEA-PHAGES; Krisanavane Reddi

doi:10.1007/978-1-0716-3798-2_17

Isolation of Bacteriophages on Actinobacteria Hosts

Methods Mol Biol. 2024:2793:273-298. doi: 10.1007/978-1-0716-3798-2_17.

Authors

Michelle Zorawik^{1

2}, Deborah Jacobs-Sera³, Amanda C Freise¹; SEA-PHAGES; Krisanavane Reddi⁴

Affiliations

¹ Department of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, CA, USA.
² Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
³ Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA, USA.
⁴ Department of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, CA, USA. krisr@microbio.ucla.edu.

PMID: 38526736
DOI: 10.1007/978-1-0716-3798-2_17

Abstract

Bacteriophages are ubiquitous biological entities which can be found in a variety of habitats. Here, we describe protocols for the isolation of bacteriophages on a variety of Actinobacterial genera. Two approaches to phage isolation, direct isolation and enriched isolation, are described, which can be performed individually or in parallel. The protocols described can be adapted to isolate a wide array of bacteriophages.

Keywords: Bacteriophage; Direct isolation; Enriched isolation; Plaques.

MeSH terms

Actinobacteria*
Bacteria
Bacteriophages* / genetics