Novel Eu-dipeptide assemblies for a fluorescence sensing strategy to ultrasensitive determine trace sulfamethazine

Rongfang Yan; Ning Zhang; Weihua Liu; Xuelian Hu; Wenxiu Wang; Yiwei Tang; Shuo Wang; Xianghong Wang; Qinghai Sheng

doi:10.1016/j.foodchem.2024.139089

Novel Eu-dipeptide assemblies for a fluorescence sensing strategy to ultrasensitive determine trace sulfamethazine

Food Chem. 2024 Aug 1:448:139089. doi: 10.1016/j.foodchem.2024.139089. Epub 2024 Mar 20.

Authors

Rongfang Yan¹, Ning Zhang¹, Weihua Liu¹, Xuelian Hu¹, Wenxiu Wang¹, Yiwei Tang², Shuo Wang³, Xianghong Wang¹, Qinghai Sheng¹

Affiliations

¹ College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China.
² College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China. Electronic address: tangyiwei81@163.com.
³ College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China; Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China.

PMID: 38518446
DOI: 10.1016/j.foodchem.2024.139089

Abstract

Self-assembled Eu-dipeptide (tryptophan-phenylalanine) microparticles with multi-emission fluorescence was prepared and modified with a single-stranded DNA corresponding to the sulfamethazine (SMZ) adapter (Eu-PMPs@cDNA). Aptamer-functionalized magnetic Fe₃O₄ (MNPs@aptamer) was used to specifically bind the target SMZ. Using Eu-PMPs@cDNA as fluorescent signal probe and MNPs@aptamer as catcher, a noncompetitive fluorescence sensing strategy was developed for determination of SMZ with good sensitivity, accuracy, selectivity, and stability. Under the optimized conditions, fluorescence increases linearly in the 0-20 ng/mL SMZ concentration range, and the detection limit is 0.014 ng/mL. The fluorescence sensing method was applied to analysis of water and fish muscle samples, and recoveries ranged from 81.78 to 119.46 % with relative standard deviations below 4.2 %. This study offered a reliable and sensitive fluorescence sensing strategy for SMZ determination in food samples, which owns great potential for wide-ranging application in harmful compounds assay by simply changing the type of aptamer and its complementary single-stranded DNA.

Keywords: Aptamer; Eu-dipeptide microparticles; Fluorescence detection; Magnetic nanoparticles; Sensing; Sulfamethazine.