Optimizing cultivation strategies and scaling up for fucoxanthin production using Pavlova sp

Chun-Yen Chen; Ping-Yung Liu; Yu-Han Chang; Dillirani Nagarajan; Mary Joy D Latagan; Mark Daniel G de Luna; Jih-Heng Chen; Jo-Shu Chang

doi:10.1016/j.biortech.2024.130609

Optimizing cultivation strategies and scaling up for fucoxanthin production using Pavlova sp

Bioresour Technol. 2024 May:399:130609. doi: 10.1016/j.biortech.2024.130609. Epub 2024 Mar 18.

Authors

Chun-Yen Chen¹, Ping-Yung Liu², Yu-Han Chang², Dillirani Nagarajan³, Mary Joy D Latagan⁴, Mark Daniel G de Luna⁵, Jih-Heng Chen⁶, Jo-Shu Chang⁷

Affiliations

¹ University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan; Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan 701, Taiwan.
² Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
³ Institute of Aquatic Science and Technology, College of Hydrosphere Science, National Kaohsiung University of Science and Technology, Nanzih Campus, Kaohsiung City, Taiwan.
⁴ Energy Engineering Program, National Graduate School of Engineering, University of the Philippines Diliman, Quezon City 1101, Philippines.
⁵ Energy Engineering Program, National Graduate School of Engineering, University of the Philippines Diliman, Quezon City 1101, Philippines; Environmental Engineering Program, National Graduate School of Engineering, University of the Philippines, Diliman, Quezon City 1101, Philippines; Department of Chemical Engineering, University of the Philippines Diliman, Quezon City 1101, Philippines.
⁶ Department of Chemical and Materials Engineering, National Kaohsiung University of Science and Technology, Kaohsiung 807, Taiwan.
⁷ Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan; Department of Chemical and Materials Engineering, Tunghai University, Taichung 407, Taiwan; Research Center for Smart Sustainable Circular Economy, Tunghai University, Taichung 407, Taiwan; Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taiwan. Electronic address: changjs@mail.ncku.edu.tw.

PMID: 38508283
DOI: 10.1016/j.biortech.2024.130609

Abstract

The microalgal-based production of fucoxanthin has emerged as an imperative research endeavor due to its antioxidant, and anticancer properties. In this study, three brown marine microalgae, namely Skeletonema costatum, Chaetoceros gracilis, and Pavlova sp., were screened for fucoxanthin production. All strains displayed promising results, with Pavlova sp. exhibiting the highest fucoxanthin content (27.91 mg/g) and productivity (1.16 mg/L·day). Moreover, the influence of various cultivation parameters, such as culture media, salinity, sodium nitrate concentration, inoculum size, light intensity, and iron concentration, were investigated and optimized, resulting in a maximum fucoxanthin productivity of 7.89 mg/L·day. The investigation was further expanded to large-scale outdoor cultivation using 50 L tubular photobioreactors, illustrating the potential of Pavlova sp. and the cultivation process for future commercialization. The biomass and fucoxanthin productivity for the large-scale cultivation were 70.7 mg/L·day and 4.78 mg/L·day, respectively. Overall, the findings demonstrated considerable opportunities for fucoxanthin synthesis via microalgae cultivation and processing.

Keywords: Light intensity; Microalgae; Nutraceuticals; Outdoor cultivation; Photobioreactor.

MeSH terms

Biomass
Haptophyta*
Light
Microalgae*
Photobioreactors
Xanthophylls

Substances

fucoxanthin
Xanthophylls