Isolation of total DNA from Placenta Samples, both Fresh and following Formalin and Paraffin Treatment

Abstract

The isolation of DNA from placental tissue suspected of infection is an important tool for identifying microorganisms such as bacteria, fungi, and viruses associated with complications during and after pregnancy. While experts primarily process placental tissue, the preservation methods employed pose challenges to extracting complete DNA. Therefore, selecting the appropriate protocol is paramount to achieving greater efficiency in obtaining genetic material.

Keywords: DNA isolation; Mechanical lysis; Placenta; Placenta in paraffin.