Fe-MOF/AuNP-based ratiometric electrochemical immunosensor for the detection of deoxynivalenol in grain products

Zaixi Shu; Huilin Hu; Zhenhong Yuan; Yue Zou; Qi Zhang; Yingli Wang; Xin Liu; Shuo Duan; Fuwei Pi; Jiahua Wang; Xiaodan Liu; Huang Dai

doi:10.1007/s00604-024-06281-9

Fe-MOF/AuNP-based ratiometric electrochemical immunosensor for the detection of deoxynivalenol in grain products

Mikrochim Acta. 2024 Mar 19;191(4):210. doi: 10.1007/s00604-024-06281-9.

Authors

Zaixi Shu^{1

2}, Huilin Hu^{1

2}, Zhenhong Yuan¹, Yue Zou¹, Qi Zhang³, Yingli Wang⁴, Xin Liu^{1

2}, Shuo Duan^{1

2}, Fuwei Pi^{1

5}, Jiahua Wang^{1

2}, Xiaodan Liu^{1

2}, Huang Dai^{6

7}

Affiliations

¹ College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.
² Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan, 430023, China.
³ School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang, 212004, China.
⁴ College of Engineering, Huazhong Agricultural University, Wuhan, 430070, China.
⁵ School of Food Science, Jiangnan University, Wuxi, 214122, China.
⁶ College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China. huangdai9@126.com.
⁷ Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan, 430023, China. huangdai9@126.com.

PMID: 38499672
DOI: 10.1007/s00604-024-06281-9

Abstract

A ratiometric assay was designed to improve the sensitivity and reliability of electrochemical immunosensors for deoxynivalenol (DON) detection. The indicator signal caused by the Fe-based metal-organic framework nanocomposites loaded with gold nanoparticles and the internal reference signal from the [Fe(CN)₆]^3-/4- in the electrolyte came together at the immunosensor. When immunoreactivity occurred, the indicator signals decreased as the concentration of DON increased, while the internal reference signals increased slightly. The ratio of the indicator signal to the internal reference signal was available for reproducible and sensitive monitoring of DON. The prepared immunosensor showed excellent performance in the range from 0.5 to 5000 pg mL^-1, and the detection limit was 0.0166 pg mL^-1. The immunosensor achieved satisfactory detection toward DON in spiked and actual samples and has a promising application in the control of DON in grain products.

Keywords: Deoxynivalenol detection; Differential pulse voltammetry; Electrochemical immunosensor; Fe-based metal–organic framework; Ratiometric.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques*
Electrochemical Techniques
Gold
Immunoassay
Metal Nanoparticles*
Reproducibility of Results
Trichothecenes*

Substances

deoxynivalenol
Gold
Trichothecenes

Grants and funding

2022YFF1102500/the National Key Research and Development Program of China