Twice-walk strategy based on three-dimensional DNA walking machine driven by duplex-specific nuclease

Sheng Lei; Zi Liu; Qidong Zhang; Zezhi Li; Gaiping Li; Lina Zou; Guobi Chai; Jian Mao; Baoxian Ye; Jianxun Zhang

doi:10.1039/d3cc06058f

Twice-walk strategy based on three-dimensional DNA walking machine driven by duplex-specific nuclease

Chem Commun (Camb). 2024 Apr 2;60(28):3778-3781. doi: 10.1039/d3cc06058f.

Authors

Sheng Lei^{1

2}, Zi Liu³, Qidong Zhang¹, Zezhi Li^{1

4}, Gaiping Li⁵, Lina Zou⁵, Guobi Chai¹, Jian Mao^{1

6}, Baoxian Ye⁵, Jianxun Zhang¹

Affiliations

¹ Zhengzhou Tobacco Research Institute of China National Tobacco Company, Zhengzhou 450001, P. R. China. jxzh258@163.com.
² College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, P. R. China.
³ Henan Key Laboratory of Biomolecular Recognition and Sensing, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu 476000, P. R. China.
⁴ School of Light Industry, Beijing Technology and Business University, Beijing 100048, P. R. China.
⁵ College of Chemistry, Zhengzhou University, Zhengzhou 450001, P. R. China.
⁶ Beijing Life Science Academy, Beijing, 102299, P. R. China.

PMID: 38494893
DOI: 10.1039/d3cc06058f

Abstract

A twice-walk strategy based on a three-dimensional (3D) cleat-equipped DNA walking machine with a high signal amplification efficiency was investigated for ultrasensitive detection of miRNA. Impressively, addition of duplex-specific nuclease (DSN) just once drove the twice-walk strategy, making the strategy simpler. With the advantages of being simple, rapid and ultrasensitive, the biosensor offers potential for use in early clinical diagnosis.

MeSH terms

Biosensing Techniques* / methods
DNA
Endonucleases
Limit of Detection
MicroRNAs* / genetics
Nucleic Acid Amplification Techniques / methods

Substances

MicroRNAs
DNA
Endonucleases