Chlorella polysaccharides have been gaining increasing attention because of their high yield from dried Chlorella powder and their remarkable immunomodulatory activity. In this study, the major polysaccharide fraction, CPP-3a, in Chlorella pyrenoidosa, was isolated, and its detailed structure was investigated by analyzing the low-molecular-weight product prepared via free radical depolymerization. The results indicated that CPP-3a with a molecular weight of 195.2 kDa was formed by →2)-α-L-Araf-(1→, →2)-α-D-Rhap-(1→, →5)-α-L-Araf-(1→, →3)-β-D-Glcp-(1→, →4)-α-D-Glcp-(1→, →4)-α-D-GlcpA-(1→, →2,3)-α-D-Manp-(1→, →3,4)-α-D-Manp-(1→, →3,4)-β-D-Galp-(1→, →3,6)-β-D-Galp-(1→, and →2,3,6)-α-D-Galp-(1→ residues, branched at C2, C3, C4, or C6 of α/β-D-Galp and α-D-Manp, and terminated by α/β-L-Araf, α-L-Arap, α-D-Galp, and β-D-Glcp. Biological assays showed that CPP-3a significantly altered the dendritic morphology of immature dendritic cells (DCs). Enhanced CD80, CD86, and MHC I expression on the cell surface and decreased phagocytic ability indicated that CPP-3a could induce the maturation of DCs. Furthermore, CPP-3a-stimulated DCs not only stimulated the proliferation of allogeneic naïve CD4+ T cells and the secretion of IFN-γ, but also directly stimulated the activation and proliferation of CD8+ T cells through cross-antigen presentation. These findings indicate that CPP-3a can promote human DC maturation and T-cell stimulation and may be a novel DC maturation inducer with potential developmental value in DC immunotherapy.
Keywords: Chlorella pyrenoidosa; D-glucopyranuronic acid (PubChem CID: 94715); D-mannopyranose (PubChem CID: 18950); Dendritic cell; Deuterium oxide (PubChem CID: 24602); Hydrogen peroxide (PubChem CID: 784); Immunomodulatory activity; L-arabinofuranose (PubChem CID: 440921); L-rhamnopyranose (PubChem CID: 439710); Lipopolysaccharide (PubChem CID: 11970143); Polysaccharide; Structure; Trifluoroacetic acid (PubChem CID: 6422); d-galactopyranose (PubChem CID: 6036); d-glucopyranose (PubChem CID: 5793).
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