High-throughput nephelometry methodology for qualitative determination of aqueous solubility of chemical libraries

Jose Brea; Maria J Varela; Geert A Daudey; Maria I Loza

doi:10.1016/j.slasd.2024.100149

High-throughput nephelometry methodology for qualitative determination of aqueous solubility of chemical libraries

SLAS Discov. 2024 Apr;29(3):100149. doi: 10.1016/j.slasd.2024.100149. Epub 2024 Mar 6.

Authors

Jose Brea¹, Maria J Varela², Geert A Daudey², Maria I Loza³

Affiliations

¹ Department of Pharmacology, Pharmacy and Pharmaceutical Technology, Centro Singular de Investigación en Medicina Molecular y Enfermedades Crónicas (CiMUS), School of Pharmacy, Universidade de Santiago de Compostela, Santiago de Compostela 15782, Spain; Health Research Institute of Santiago de Compostela (IDIS), University Hospital of Santiago de Compostela (SERGAS), Trav. Choupana s/n, Santiago de Compostela 15706, Spain. Electronic address: pepo.brea@usc.es.
² Department of Pharmacology, Pharmacy and Pharmaceutical Technology, Centro Singular de Investigación en Medicina Molecular y Enfermedades Crónicas (CiMUS), School of Pharmacy, Universidade de Santiago de Compostela, Santiago de Compostela 15782, Spain.
³ Department of Pharmacology, Pharmacy and Pharmaceutical Technology, Centro Singular de Investigación en Medicina Molecular y Enfermedades Crónicas (CiMUS), School of Pharmacy, Universidade de Santiago de Compostela, Santiago de Compostela 15782, Spain; Health Research Institute of Santiago de Compostela (IDIS), University Hospital of Santiago de Compostela (SERGAS), Trav. Choupana s/n, Santiago de Compostela 15706, Spain. Electronic address: mabel.loza@usc.es.

PMID: 38492994
DOI: 10.1016/j.slasd.2024.100149

Abstract

The purpose of the protocol reported in this work is the solubility profiling of large chemical libraries using nephelometry. This technique allows the qualitative classification of compounds as highly, moderately, or poorly water-soluble. The described methodology is not intended to yield quantitative solubility values of the studied compounds but can be used as a primary solubility assessment of large chemical libraries, to guide hit prioritization after High Throughput Screening (HTS) campaigns.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

High-Throughput Screening Assays* / methods
Nephelometry and Turbidimetry / methods
Small Molecule Libraries* / chemistry
Solubility*
Water* / chemistry

Substances

Small Molecule Libraries
Water