PCNA cycling dynamics during DNA replication and repair in mammals

Sukhyun Kang; Juyeong Yoo; Kyungjae Myung

doi:10.1016/j.tig.2024.02.006

PCNA cycling dynamics during DNA replication and repair in mammals

Trends Genet. 2024 Mar 13:S0168-9525(24)00042-8. doi: 10.1016/j.tig.2024.02.006. Online ahead of print.

Authors

Sukhyun Kang¹, Juyeong Yoo², Kyungjae Myung³

Affiliations

¹ Center for Genomic Integrity, Institute for Basic Science (IBS), Ulsan 44919, Republic of Korea.
² Center for Genomic Integrity, Institute for Basic Science (IBS), Ulsan 44919, Republic of Korea; Department of Biological Sciences, Ulsan National Institute of Science and Technology, Ulsan 44919, Republic of Korea.
³ Center for Genomic Integrity, Institute for Basic Science (IBS), Ulsan 44919, Republic of Korea; Department of Biomedical Engineering, Ulsan National Institute of Science and Technology, Ulsan 44919, Republic of Korea. Electronic address: kmyung@ibs.re.kr.

PMID: 38485608
DOI: 10.1016/j.tig.2024.02.006

Abstract

Proliferating cell nuclear antigen (PCNA) is a eukaryotic replicative DNA clamp. Furthermore, DNA-loaded PCNA functions as a molecular hub during DNA replication and repair. PCNA forms a closed homotrimeric ring that encircles the DNA, and association and dissociation of PCNA from DNA are mediated by clamp-loader complexes. PCNA must be actively released from DNA after completion of its function. If it is not released, abnormal accumulation of PCNA on chromatin will interfere with DNA metabolism. ATAD5 containing replication factor C-like complex (RLC) is a PCNA-unloading clamp-loader complex. ATAD5 deficiency causes various DNA replication and repair problems, leading to genome instability. Here, we review recent progress regarding the understanding of the action mechanisms of PCNA unloading complex in DNA replication/repair pathways.

Keywords: ATAD5; DNA repair; DNA replication; PCNA; RFC.

Publication types

Review