Generation of a TBX20 homozygous knockout stem cell line (WAe009-A-1E) by episomal vector-based CRISPR/Cas9 system

Xiaojie Hou; Junsheng Mu

doi:10.1016/j.scr.2024.103384

Generation of a TBX20 homozygous knockout stem cell line (WAe009-A-1E) by episomal vector-based CRISPR/Cas9 system

Stem Cell Res. 2024 Jun:77:103384. doi: 10.1016/j.scr.2024.103384. Epub 2024 Mar 11.

Authors

Xiaojie Hou¹, Junsheng Mu²

Affiliations

¹ Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100069, China. Electronic address: jaysonhou@163.com.
² Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100069, China. Electronic address: wesleymu@ccmu.edu.cn.

PMID: 38479330
DOI: 10.1016/j.scr.2024.103384

Abstract

The T-box family transcription factor gene TBX20 plays a crucial role in cardiac development and function. TBX20 mutations are associated with congenital heart disease, dilated cardiomyopathy, arrhythmias, and heart failure. To further study the role of TBX20 in human heart, here we generated a homozygous TBX20 knockout (TBX20-KO) human embryonic stem cell line using the CRISPR/Cas9 system. This TBX20-KO cell line maintains normal morphology, pluripotency, and karyotype, making it a valuable tool for investigating TBX20's role in cardiac biology.

MeSH terms

CRISPR-Cas Systems*
Cell Line
Gene Knockout Techniques
Genetic Vectors / metabolism
Homozygote
Human Embryonic Stem Cells / cytology
Human Embryonic Stem Cells / metabolism
Humans
Plasmids / genetics
Plasmids / metabolism
T-Box Domain Proteins* / genetics
T-Box Domain Proteins* / metabolism

Substances

T-Box Domain Proteins
TBX20 protein, human