A Method for Screening a Kinase Inhibitor Drug Library on ASCs

Caroline H Rinderle; Bruce A Bunnell

doi:10.1007/978-1-0716-3762-3_21

A Method for Screening a Kinase Inhibitor Drug Library on ASCs

Methods Mol Biol. 2024:2783:303-308. doi: 10.1007/978-1-0716-3762-3_21.

Authors

Caroline H Rinderle¹, Bruce A Bunnell²

Affiliations

¹ Department of Microbiology, Immunology and Genetics, School of Biomedical Sciences, University of North Texas Health Science Center, Fort Worth, TX, USA.
² Department of Microbiology, Immunology and Genetics, School of Biomedical Sciences, University of North Texas Health Science Center, Fort Worth, TX, USA. Bruce.Bunnell@unthsc.edu.

PMID: 38478242
DOI: 10.1007/978-1-0716-3762-3_21

Abstract

Breast cancer is an ongoing issue due to its high mortality rates. Obesity enhances the problems associated with breast cancer, meaning there must be a biological connection between them. This crosstalk may be the adipose-derived stem cell. If we can interrupt the communication between adipose-derived stromal/stem cells (ASCs) and breast cancer, we may be able to prevent cancer propagation. Specific kinase inhibition may allow us to downregulate signals, preventing ASC-mediated cancer growth. This chapter provides a critical method for screening a kinase inhibitor drug library for hits on ASCs.

Keywords: Adipose stem cells; Drug screen; Inhibitors; kinase.

MeSH terms

Adipocytes
Adipose Tissue*
Breast Neoplasms* / drug therapy
Cell Proliferation
Female
Humans
Obesity
Stromal Cells / physiology