Preparation of Decellularized Amniotic Membrane and Adipose-Derived Stromal/Stem Cell Seeding

Haadia Tauseef; Kainat Ahmed; Asmat Salim; Omair A Mohiuddin

doi:10.1007/978-1-0716-3762-3_14

Preparation of Decellularized Amniotic Membrane and Adipose-Derived Stromal/Stem Cell Seeding

Methods Mol Biol. 2024:2783:209-220. doi: 10.1007/978-1-0716-3762-3_14.

Authors

Haadia Tauseef¹, Kainat Ahmed¹, Asmat Salim¹, Omair A Mohiuddin²

Affiliations

¹ Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan.
² Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan. omair.mohiuddin@iccs.edu.

PMID: 38478235
DOI: 10.1007/978-1-0716-3762-3_14

Abstract

Amniotic membrane, being part of the placenta, is discarded as medical waste after childbirth. It can be decellularized to convert it into an acellular material while retaining the extracellular matrix. Such amniotic membrane grafts support stem cell adhesion, growth, and proliferation. These properties make it a useful candidate to be used as a bio-scaffold in regenerative medicine. This chapter describes a method for the decellularization of the amniotic membrane. Furthermore, the method for seeding adipose-derived stem cells on the decellularized amniotic membrane is described.

Keywords: Adipose-derived stromal/stem cells; Cell seeding; DNA extraction; Decellularized human amniotic membrane; Histology; Silicone sheet.

MeSH terms

Adipocytes
Amnion*
Extracellular Matrix / metabolism
Stem Cells
Tissue Engineering / methods
Tissue Scaffolds*