Adult Stem Cells Freezing Processes and Cryopreservation Protocols

Mohan Kumar Dey; Ram V Devireddy

doi:10.1007/978-1-0716-3762-3_5

Adult Stem Cells Freezing Processes and Cryopreservation Protocols

Methods Mol Biol. 2024:2783:53-89. doi: 10.1007/978-1-0716-3762-3_5.

Authors

Mohan Kumar Dey¹, Ram V Devireddy²

Affiliations

¹ Bioengineering Laboratory, Department of Mechanical Engineering, Louisiana State University, Baton Rouge, LA, USA.
² Bioengineering Laboratory, Department of Mechanical Engineering, Louisiana State University, Baton Rouge, LA, USA. devireddy@me.lsu.edu.

PMID: 38478226
DOI: 10.1007/978-1-0716-3762-3_5

Abstract

The development of simple but effective storage protocols for adult stem cells will greatly enhance their use and utility in tissue-engineering applications. Cryopreservation has shown the most promise but is a fairly complex process, necessitating the use of chemicals called cryoprotective agents (CPAs), freezing equipment, and obviously, storage in liquid nitrogen. The purpose of this chapter is to present a general overview of cryopreservation storage techniques and the optimal protocols/results obtained in our laboratory for long-term storage of adult stem cells using freezing storage.

Keywords: Adipogenesis; Apoptosis; Cryopreservation; Cryoprotective agents; Dimethylsulfoxide; Flow cytometry; Histo-chemical analysis; Osteogenesis; Polyvinylpyrrolidone.

Publication types

Review

MeSH terms

Adipose Tissue
Adult
Adult Stem Cells*
Cell Survival
Cryopreservation* / methods
Cryoprotective Agents / pharmacology
Freezing
Humans

Substances

Cryoprotective Agents