A Transparent and Injectable Biomaterial Prepared by Mixing Collagen and Anti-Cancer Platinum Derivatives

Shinichiro F Ichise; Takaki Koide

doi:10.1002/mabi.202300553

A Transparent and Injectable Biomaterial Prepared by Mixing Collagen and Anti-Cancer Platinum Derivatives

Macromol Biosci. 2024 Mar 9:e2300553. doi: 10.1002/mabi.202300553. Online ahead of print.

Authors

Shinichiro F Ichise^{1

2}, Takaki Koide^{2

3}

Affiliations

¹ Department of Clinical Nutrition, Kitasato Junior College of Health and Hygienic Sciences, Niigata, 949-7241, Japan.
² Waseda Research Institute for Science and Engineering, Tokyo, 169-8555, Japan.
³ Department of Chemistry and Biochemistry, School of Advanced Science and Engineering, Waseda University, Tokyo, 169-8555, Japan.

PMID: 38459799
DOI: 10.1002/mabi.202300553

Abstract

This study presents the synthesis of a cross-linked collagen material, named platinum-containing collagen gel (PCG), which is achieved by simply mixing collagen and derivatives of an anti-cancer platinum complex. The cross-linking reagents are derivatives of cisplatin or transplatin, generated through a ligand exchange with dimethyl sulfoxide. PCG exhibits superior physical strength and transparency compared with the native collagen gel formed through spontaneous fibril formation. The versatility of PCG as a cell culture scaffold, applicable to both 2D and 3D models, with low cytotoxicity is demonstrated. Furthermore, PCG exhibits pH-responsive gel-forming properties. This enables the removal of free cross-linker by dialysis in an acidic solution and subsequent gel formation upon neutralization. This material holds promise for application in cell culture scaffolds and medical injections.

Keywords: 3D cell culture; collagen; hydrogel; injectable biomaterial.