The performance of a Fully Automated Urine Particle Analyzer, Sysmex UF-5000, in detecting fastidious bacteria in urine samples

Masako Kaido; Mitsuru Yasuda; Masahiro Hayashi; Hazuki Ohashi; Hirotoshi Ohta; Yasumasa Akai; Kaori Tanaka; Takashi Deguchi

doi:10.1016/j.mimet.2024.106913

The performance of a Fully Automated Urine Particle Analyzer, Sysmex UF-5000, in detecting fastidious bacteria in urine samples

J Microbiol Methods. 2024 May:220:106913. doi: 10.1016/j.mimet.2024.106913. Epub 2024 Mar 6.

Authors

Masako Kaido¹, Mitsuru Yasuda², Masahiro Hayashi³, Hazuki Ohashi⁴, Hirotoshi Ohta⁵, Yasumasa Akai⁶, Kaori Tanaka⁷, Takashi Deguchi⁸

Affiliations

¹ Medical & Scientific Affairs, Sysmex Corporation, 4-4-4 Takatsukadai, Nisi-ku, Kobe, Hyogo 651-2271, Japan. Electronic address: kaido.masako@sysmex.co.jp.
² Department of Infection Control and Laboratory Medicine, Sapporo Medical University of Medicine, S1 W16, Chuo-ku, Sapporo, Hokkaido 060-8543, Japan. Electronic address: yasuda7jpe@sapmed.ac.jp.
³ Center for Conservation of Microbial Genetic Resource, Gifu University, 1-1 Yanagido, Gifu city, Gifu 501-1194, Japan; Division of Anaerobe Research, Integrated Glyco-Molecular Science Research Center, Institute for Glyco-core Research, Gifu University, 1-1 Yanagido, Gifu City, Gifu 501-1194, Japan. Electronic address: hayashi.masahiro.w8@f.gifu-u.ac.jp.
⁴ Division of Clinical Laboratory, Gifu University Hospital, Gifu University, Gifu City, Gifu, 501-1194, Japan. Electronic address: hazuoha@gifu-u.ac.jp.
⁵ Division of Clinical Laboratory, Gifu University Hospital, Gifu University, Gifu City, Gifu, 501-1194, Japan. Electronic address: oota.hirotoshi.041@mail.aichi-med-u.ac.jp.
⁶ Regulatory Affairs & Quality Assurance, Sysmex Corporation, 1-3-2 Murotani, Nisi-ku, Kobe, Hyogo 651-2241, Japan. Electronic address: Akai.Yasumasa@sysmex.co.jp.
⁷ Center for Conservation of Microbial Genetic Resource, Gifu University, 1-1 Yanagido, Gifu city, Gifu 501-1194, Japan; Division of Anaerobe Research, Integrated Glyco-Molecular Science Research Center, Institute for Glyco-core Research, Gifu University, 1-1 Yanagido, Gifu City, Gifu 501-1194, Japan. Electronic address: tanaka.kaori.y7@f.gifu-u.ac.jp.
⁸ Department of Urology, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu City, Gifu 501-1193, Japan; Department of Urology, Kizawa Memorial Hospital, 590 Shimokobi, Kobicho, Minokamo, Gifu 505-8503, Japan.

PMID: 38458394
DOI: 10.1016/j.mimet.2024.106913

Abstract

Several types of fastidious bacteria can cause tract infections. We evaluated the performance of counting fastidious bacteria using a Fully Automated Urine Particle Analyzer UF-5000. The results showed that UF-5000 counts fastidious bacteria in urine without the need for culture using measurement principles based on flow cytometry.

Keywords: Fastidious bacteria; Flowcytometry; Fully automated urine particle analyzer UF-5000; Urinary tract infections.

MeSH terms

Bacteria
Flow Cytometry / methods
Humans
Urinary Tract Infections* / microbiology
Urine / microbiology