The effects of ultrasonic processing on the antioxidant activity of Geotrichum candidum LG-8 and its cell wall extracts

Fengping Jiao; Xianping Cui; Xiaodi Gong; Guozheng Jiang; Jinbiao Wang; Ling Meng

doi:10.3389/fnut.2024.1334956

The effects of ultrasonic processing on the antioxidant activity of Geotrichum candidum LG-8 and its cell wall extracts

Front Nutr. 2024 Feb 21:11:1334956. doi: 10.3389/fnut.2024.1334956. eCollection 2024.

Authors

Fengping Jiao^#¹, Xianping Cui^#², Xiaodi Gong¹, Guozheng Jiang³, Jinbiao Wang³, Ling Meng¹

Affiliations

¹ School of Public Health, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, China.
² Division of Hepatobiliary and Pancreatic Surgery, Affiliated Provincial Hospital, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, China.
³ Yantai Hengyuan Bioengineering Co. Ltd., Yantai, Shandong, China.

^# Contributed equally.

Abstract

Introduction: Extraction techniques that influence cell wall polysaccharides (EPS) is crucial for maximizing their bioactivity. This study evaluates ultrasound technology for extracting antioxidant polysaccharides from Geotrichum candidum LG-8, assessing its impacton antioxidant activity.

Methods: Ultrasound extraction of EPS from G. candidum LG-8 was optimized (18 min, pH 7.0, 40 W/cm², 0.75 M NaCl). ABTS scavenging efficiency and monosaccharide composition of LG-EPS1 and LG-EPS3 were analyzed using Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM).

Results: The Results showed that ultrasonic treatment markedly increased the ABTS radical scavenging efficiency of LG-8 cells by 47%. At a concentration of 1 mg/mL, the ultrasonically extracted LG-EPS1 and LG-EPS3 polysaccharides exhibited significant ABTS radical scavenging efficiencies of 26% and 51%, respectively. Monosaccharide composition analysis identified mannose and glucose in LG-EPS1, while LG-EPS3 was primarily composed of mannose. FTIR spectra verified the polysaccharides' presence, and SEM provided visual confirmation of the nanoparticle structures characteristic of LG-EPS1 and LG-EPS3.

Discussion: This research not only underscores the technological merits of ultrasound in polysaccharide extraction but also highlights the potential of G. candidum LG-8 derived polysaccharides as valuable bioactive compounds for antioxidant utilization.

Keywords: ABTS; Geotrichum; antioxidant; nanopolysaccharide; ultrasonic processing.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by the Tai'an Science and Technology Innovation Development Project (2021NS186), TCM Science and Technology Project of Shandong Province (M-2022260), Natural Science Foundation of Shandong Province (ZR2023MC151), Young People Fund of Shandong Academy of Medical Sciences (202201-030, 2022104391658, 2022104391089, and 2022104391616), and Postdoctoral program from Yantai Hengyuan Bioengineering Co., Ltd.