A Lipidomics Approach to Determine the Role of Lipids and Its Crosstalk with Autophagy in Lung Cancer Metastasis

Simone C da Silva Rosa; Javad Alizadeh; Rui Vitorino; Arun Surendran; Amir Ravandi; Biniam Kidane; Saeid Ghavami

doi:10.1007/7651_2024_524

A Lipidomics Approach to Determine the Role of Lipids and Its Crosstalk with Autophagy in Lung Cancer Metastasis

Methods Mol Biol. 2024 Mar 6. doi: 10.1007/7651_2024_524. Online ahead of print.

Authors

Simone C da Silva Rosa¹, Javad Alizadeh¹, Rui Vitorino^{2

3}, Arun Surendran⁴, Amir Ravandi⁵, Biniam Kidane⁶, Saeid Ghavami^{7

8

9}

Affiliations

¹ Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada.
² Department of Surgery and Physiology, Faculty of Medicine, University of Porto, Porto, Portugal.
³ Department of Medical Sciences, Institute of Biomedicine iBiMED, University of Aveiro, Aveiro, Portugal.
⁴ Mass Spectrometry Core Facility, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India.
⁵ Department of Physiology and Pathophysiology, Max Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada.
⁶ Department of Surgery, Max Rady College of Medicine, University of Manitoba, Winnipeg, MB, Canada.
⁷ Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada. saeid.ghavami@umanitoba.ca.
⁸ Faculty of Medicine in Zabrze, University of Technology in Katowice, Zabrze, Poland. saeid.ghavami@umanitoba.ca.
⁹ Research Institute of Oncology and Hematology, Cancer Care Manitoba, University of Manitoba, Winnipeg, MB, Canada. saeid.ghavami@umanitoba.ca.

PMID: 38441721
DOI: 10.1007/7651_2024_524

Abstract

Non-small cell lung cancer (NSCLC) is among the most malignant tumors with high propensity for metastasis and is the leading cause of cancer-related death globally. Most patients present with regional and distant metastasis, associated with poor prognosis. Lipids may play an essential role in either activating or inhibiting detachment-induced apoptosis (anoikis), where the latter is a crucial mechanism to prevent metastasis, and it may have a cross-talk with autophagy. Autophagy has been shown to be induced in various human cancer metastasis, modulating tumor cell motility and invasion, cancer cell differentiation, resistance to anoikis, and epithelial to mesenchymal transition. Hence, it may play a crucial role in the transition of benign to malignant phenotypes, the core of metastasis initiation. Here, we provide a method we have established in our laboratory for detecting lipids in attached and detached non-small lung cancer cells and show how to analyze lipidomics data to find its correlation with autophagy-related pathways.

Keywords: Anoikis; Autophagy; Lipidomics; Lung cancer; Mass spectrometry; MetaboAnalyst; Metastasis.