Friends without benefits: Extensive cytotype sympatry and polyploid persistence in an African geophyte

Damian Vaz de Sousa; Michelle Greve; Kenneth C Oberlander

doi:10.1002/ajb2.16291

Friends without benefits: Extensive cytotype sympatry and polyploid persistence in an African geophyte

Am J Bot. 2024 Mar 4:e16291. doi: 10.1002/ajb2.16291. Online ahead of print.

Authors

Damian Vaz de Sousa^{1

2}, Michelle Greve¹, Kenneth C Oberlander²

Affiliations

¹ Department of Plant and Soil Science, University of Pretoria, Private Bag X20, Hatfield, 0028, Pretoria, South Africa.
² H.G.W.J. Schweickerdt Herbarium, Department of Plant and Soil Science, University of Pretoria, Pretoria, South Africa.

PMID: 38439133
DOI: 10.1002/ajb2.16291

Abstract

Premise: Polyploidy is a major factor in plant adaptation and speciation. Multiple mechanisms contribute to autopolyploid frequency within populations, but uncertainties remain regarding mechanisms that facilitate polyploid establishment and persistence. Here we aimed to document and predict cytotype distributions of Oxalis obliquifolia Steud. ex A. Rich. across Gauteng, South Africa, and test for evidence of possible mechanisms, including morphological, phenological, and reproductive traits, that may potentially facilitate polyploid persistence.

Methods: Over 320 O. obliquifolia plants from 25 sites were cytotyped using flow cytometry, and DNA ploidy was confirmed using meiotic chromosome squashes. Cytotypes were mapped and correlations with abiotic variables assessed using ordinations. To assess morphological and phenological associations with cytotype, we grew multiple cytotypes in a common garden, measured phenotypic traits and compared them using linear models and discriminant analyses. Intercytotype reproductive isolation was assessed using crossing experiments, and AMOVAs based on ITS DNA sequences tested for cytogeographic structure.

Results: Six cytotypes were identified, and most sites had multiple cytotypes. Abiotic variables were not predictive of cytotype distribution. A clear gigas effect was present. Differences in flower size and phenology suggested pollinator interactions could play a role in polyploid persistence. Intercytotype crosses produced seed at low frequency. DNA data suggested diploids and polyploids were largely reproductively isolated in situ, and polyploidization events were not frequent enough to explain high cytotype sympatry.

Conclusions: Diploids and polyploids are behaving as separate species, despite little observable niche differentiation and non-zero potential intercytotype seed set. Tests on biotic interactions and intercytotype F1 fitness may provide insights into diploid and polyploid coexistence.

Keywords: Oxalidaceae; autopolyploidy; cytogeography; cytotype sympatry; minority cytotype exclusion; polyploid persistence; whole genome duplication.