Bi-allelic variants in CELSR3 are implicated in central nervous system and urinary tract anomalies

Jil D Stegmann; Jeshurun C Kalanithy; Gabriel C Dworschak; Nina Ishorst; Enrico Mingardo; Filipa M Lopes; Yee Mang Ho; Phillip Grote; Tobias T Lindenberg; Öznur Yilmaz; Khadija Channab; Steve Seltzsam; Shirlee Shril; Friedhelm Hildebrandt; Felix Boschann; André Heinen; Angad Jolly; Katherine Myers; Kim McBride; Mir Reza Bekheirnia; Nasim Bekheirnia; Marcello Scala; Manuela Morleo; Vincenzo Nigro; Annalaura Torella; TUDP consortium; Michele Pinelli; Valeria Capra; Andrea Accogli; Silvia Maitz; Alice Spano; Rory J Olson; Eric W Klee; Brendan C Lanpher; Se Song Jang; Jong-Hee Chae; Philipp Steinbauer; Dietmar Rieder; Andreas R Janecke; Julia Vodopiutz; Ida Vogel; Jenny Blechingberg; Jennifer L Cohen; Kacie Riley; Victoria Klee; Laurence E Walsh; Matthias Begemann; Miriam Elbracht; Thomas Eggermann; Arzu Stoppe; Kyra Stuurman; Marjon van Slegtenhorst; Tahsin Stefan Barakat; Maureen S Mulhern; Tristan T Sands; Cheryl Cytrynbaum; Rosanna Weksberg; Federica Isidori; Tommaso Pippucci; Giulia Severi; Francesca Montanari; Michael C Kruer; Somayeh Bakhtiari; Hossein Darvish; Heiko Reutter; Gregor Hagelueken; Matthias Geyer; Adrian S Woolf; Jennifer E Posey; James R Lupski; Benjamin Odermatt; Alina C Hilger

doi:10.1038/s41525-024-00398-9

Bi-allelic variants in CELSR3 are implicated in central nervous system and urinary tract anomalies

NPJ Genom Med. 2024 Mar 1;9(1):18. doi: 10.1038/s41525-024-00398-9.

Authors

Jil D Stegmann^#^{1

2}, Jeshurun C Kalanithy^#^{3

4}, Gabriel C Dworschak^{3

4

5}, Nina Ishorst^{3

4}, Enrico Mingardo⁶, Filipa M Lopes⁷, Yee Mang Ho⁷, Phillip Grote⁸, Tobias T Lindenberg⁴, Öznur Yilmaz⁴, Khadija Channab⁶, Steve Seltzsam⁹, Shirlee Shril⁹, Friedhelm Hildebrandt⁹, Felix Boschann¹⁰, André Heinen¹¹, Angad Jolly^{12

13}, Katherine Myers¹⁴, Kim McBride¹⁴, Mir Reza Bekheirnia^{12

15}, Nasim Bekheirnia^{15

16}, Marcello Scala^{17

18}, Manuela Morleo^{19

20}, Vincenzo Nigro^{19

20}, Annalaura Torella^{19

20}; TUDP consortium; Michele Pinelli^{20

21}, Valeria Capra²², Andrea Accogli^{23

24}, Silvia Maitz²⁵, Alice Spano²⁶, Rory J Olson²⁷, Eric W Klee^{27

28

29}, Brendan C Lanpher^{27

28}, Se Song Jang³⁰, Jong-Hee Chae^{30

31}, Philipp Steinbauer³², Dietmar Rieder³³, Andreas R Janecke^{34

35}, Julia Vodopiutz³⁶, Ida Vogel^{37

38}, Jenny Blechingberg³⁸, Jennifer L Cohen³⁹, Kacie Riley⁴⁰, Victoria Klee⁴¹, Laurence E Walsh⁴¹, Matthias Begemann⁴², Miriam Elbracht⁴², Thomas Eggermann⁴², Arzu Stoppe⁴³, Kyra Stuurman⁴⁴, Marjon van Slegtenhorst⁴⁴, Tahsin Stefan Barakat⁴⁴, Maureen S Mulhern^{45

46}, Tristan T Sands^{47

48

49}, Cheryl Cytrynbaum^{50

51}, Rosanna Weksberg^{51

52}, Federica Isidori⁵³, Tommaso Pippucci⁵³, Giulia Severi⁵³, Francesca Montanari⁵³, Michael C Kruer^{54

55}, Somayeh Bakhtiari^{54

55}, Hossein Darvish⁵⁶, Heiko Reutter^{3

57

58}, Gregor Hagelueken⁵⁹, Matthias Geyer⁵⁹, Adrian S Woolf^{7

60}, Jennifer E Posey¹², James R Lupski^{12

16

61

62}, Benjamin Odermatt^{6

4}, Alina C Hilger^{63

64}

Affiliations

¹ Institute of Human Genetics, Medical Faculty, University of Bonn, Bonn, 53127, Germany. jil.stegmann@uni-bonn.de.
² Institute of Anatomy and Cell Biology, Medical Faculty, University of Bonn, Bonn, 53115, Germany. jil.stegmann@uni-bonn.de.
³ Institute of Human Genetics, Medical Faculty, University of Bonn, Bonn, 53127, Germany.
⁴ Institute of Neuroanatomy, Medical Faculty, University of Bonn, Bonn, 53115, Germany.
⁵ Department of Neuropediatrics, University Hospital Bonn, Bonn, 53127, Germany.
⁶ Institute of Anatomy and Cell Biology, Medical Faculty, University of Bonn, Bonn, 53115, Germany.
⁷ Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, University of Manchester, Manchester, UK.
⁸ Georg-Speyer-Haus, Institute for Tumor Biology and Experimental Therapy, 60596, Frankfurt am Main, Germany.
⁹ Division of Nephrology, Department of Pediatrics, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA.
¹⁰ Institute of Medical Genetics and Human Genetics, Charité Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.
¹¹ Department of Pediatrics, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
¹² Department of Molecular & Human Genetics, Baylor College of Medicine, Houston, TX, 77030, USA.
¹³ Medical Scientist Training Program, Baylor College of Medicine, Houston, TX, USA.
¹⁴ Center for Cardiovascular Research, Nationwide Children's Hospital, Department of Pediatrics, Ohio State University, Columbus, OH, USA.
¹⁵ Department of Pediatrics, Renal Service, Texas Children's Hospital, Houston, TX, 77030, USA.
¹⁶ Department of Pediatrics, Baylor College of Medicine, Houston, TX, 77030, USA.
¹⁷ Department of Neurosciences, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (DINOGMI), University of Genoa, 16132, Genoa, Italy.
¹⁸ U.O.C. Genetica Medica, IRCCS Istituto Giannina Gaslini, 16147, Genoa, Italy.
¹⁹ Medical Genetics, Department of Precision Medicine, Università degli Studi della Campania 'Luigi Vanvitelli', via Luigi De Crecchio 7, 80138, Naples, Italy.
²⁰ Telethon Institute of Genetics and Medicine, Pozzuoli, Naples, Italy.
²¹ Department of Molecular Medicine and Medical Biotechnologies, University Federico II, Naples, Italy.
²² Genomics and Clinical Genetics, IRCCS Gaslini, Genoa, Italy.
²³ Division of Medical Genetics, Department of Specialized Medicine, McGill University, Montreal, QC, Canada.
²⁴ Department of Human Genetics, McGill University, Montreal, QC, Canada.
²⁵ Medical Genetics Service, Oncology Department of Southern Switzerland, Ente Ospedaliero Cantonale, Lugano, Switzerland.
²⁶ MBBM Foundation, Monza, Italy.
²⁷ Center for Individualized Medicine, Mayo Clinic, Rochester, MN, USA.
²⁸ Department of Clinical Genomics, Mayo Clinic, Rochester, MN, USA.
²⁹ Department of Quantitative Health Sciences, Mayo Clinic, Rochester, MN, USA.
³⁰ Department of Pediatrics, Seoul National University College of Medicine, Seoul, Republic of Korea.
³¹ Department of Genomics Medicine, Rare Disease Center, Seoul National University Hospital, Seoul, Republic of Korea.
³² Division of Neonatology, Pediatric Intensive Care and Neuropediatrics, Comprehensive Center for Pediatrics, Medical University of Vienna, Vienna, Austria.
³³ Division of Bioinformatics, Medical University of Innsbruck, 6020, Innsbruck, Austria.
³⁴ Department of Pediatrics I, Medical University of Innsbruck, 6020, Innsbruck, Austria.
³⁵ Division of Human Genetics, Medical University of Innsbruck, 6020, Innsbruck, Austria.
³⁶ Department of Pediatrics and Adolescent Medicine, Division of Pediatric Pulmonology, Allergology and Endocrinology, Comprehensive Center for Pediatrics, Medical University of Vienna, 1090, Vienna, Austria.
³⁷ Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.
³⁸ Department of Clinical Genetics, Aarhus University Hospital, Aarhus, Denmark.
³⁹ Division of Medical Genetics, Department of Pediatrics, Duke University, Durham, NC, USA.
⁴⁰ Department of Pediatrics, Duke University Medical Center, Durham, NC, USA.
⁴¹ Pediatric Neurology, Riley Hospital for Children Indiana University Health, Indianapolis, IN, USA.
⁴² Institute for Human Genetics and Genomic Medicine, Medical Faculty, RWTH Aachen University, Aachen, Germany.
⁴³ Division of Neuropediatrics and Social Pediatrics, Department of Pediatrics, Medical Faculty, RWTH Aachen University, 52074, Aachen, Germany.
⁴⁴ Department of Clinical Genetics, Erasmus MC University Medical Center, Rotterdam, The Netherlands.
⁴⁵ Department of Neurology, Columbia University Vagelos College of Physicians and Surgeons, New York, NY, USA.
⁴⁶ Department of Pathology, Columbia University Vagelos College of Physicians and Surgeons, New York, NY, USA.
⁴⁷ Division of Child Neurology, Department of Neurology, Columbia University Vagelos College of Physicians and Surgeons and NewYork-Presbyterian Morgan Stanley Children's Hospital, New York, NY, USA.
⁴⁸ Department of Pediatrics, Columbia University Vagelos College of Physicians and Surgeons and NewYork-Presbyterian Morgan Stanley Children's Hospital, New York, NY, USA.
⁴⁹ Institute for Genomic Medicine, Columbia University Vagelos College of Physicians and Surgeons, New York, NY, USA.
⁵⁰ Department of Genetic Counselling, The Hospital for Sick Children, Toronto, ON, M5G 1X8, Canada.
⁵¹ Department of Molecular Genetics, University of Toronto, Toronto, ON, M5S 1A1, Canada.
⁵² Division of Clinical and Metabolic Genetics, The Hospital for Sick Children, Toronto, ON, M5G 1X8, Canada.
⁵³ U.O. Genetica Medica, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy.
⁵⁴ Pediatric Movement Disorders Program, Division of Pediatric Neurology, Barrow Neurological Institute, Phoenix Children's Hospital, Phoenix, AZ, USA.
⁵⁵ Departments of Child Health, Neurology, and Cellular & Molecular Medicine, and Program in Genetics, University of Arizona College of Medicine-Phoenix, Phoenix, AZ, USA.
⁵⁶ Neuroscience Research Center, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
⁵⁷ Division Neonatology and Pediatric Intensive Care, Department of Pediatric and Adolescent Medicine, Friedrich-Alexander University of Erlangen-Nürnberg, Erlangen, Germany.
⁵⁸ Institute of Human Genetics, Friedrich-Alexander University of Erlangen-Nürnberg, Erlangen, Germany.
⁵⁹ Institute of Structural Biology, University Hospital Bonn, University of Bonn, Venusberg-Campus 1, 53127, Bonn, Germany.
⁶⁰ Royal Manchester Children's Hospital, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.
⁶¹ Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX, 77030, USA.
⁶² Texas Children's Hospital, Houston, TX, 77030, USA.
⁶³ Department of Pediatric and Adolescent Medicine, Friedrich-Alexander University of Erlangen-Nürnberg, Erlangen, 91054, Germany. alina.hilger@uk-erlangen.de.
⁶⁴ Research Center On Rare Kidney Diseases (RECORD), University Hospital Erlangen, 91054, Erlangen, Germany. alina.hilger@uk-erlangen.de.

^# Contributed equally.

Abstract

CELSR3 codes for a planar cell polarity protein. We describe twelve affected individuals from eleven independent families with bi-allelic variants in CELSR3. Affected individuals presented with an overlapping phenotypic spectrum comprising central nervous system (CNS) anomalies (7/12), combined CNS anomalies and congenital anomalies of the kidneys and urinary tract (CAKUT) (3/12) and CAKUT only (2/12). Computational simulation of the 3D protein structure suggests the position of the identified variants to be implicated in penetrance and phenotype expression. CELSR3 immunolocalization in human embryonic urinary tract and transient suppression and rescue experiments of Celsr3 in fluorescent zebrafish reporter lines further support an embryonic role of CELSR3 in CNS and urinary tract formation.

Abstract

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