Prevotella, a dominant bacterium in young people with stage Ⅲ periodontitis, related to the arachidonic acid metabolism pathway

Qin Ye; Yaqiong Zhao; Jie Zhao; Zeyue Ouyang; Yao Feng; Jing Hu; Xiaolin Su; Ningxin Chen; Yun Chen; Li Tan; Yunzhi Feng; Yue Guo

doi:10.1016/j.micinf.2024.105316

Prevotella, a dominant bacterium in young people with stage Ⅲ periodontitis, related to the arachidonic acid metabolism pathway

Microbes Infect. 2024 Feb 27:105316. doi: 10.1016/j.micinf.2024.105316. Online ahead of print.

Authors

Qin Ye¹, Yaqiong Zhao¹, Jie Zhao¹, Zeyue Ouyang¹, Yao Feng¹, Jing Hu¹, Xiaolin Su¹, Ningxin Chen¹, Yun Chen¹, Li Tan¹, Yunzhi Feng², Yue Guo³

Affiliations

¹ Department of Stomatology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China; Hunan Provincial Engineering Research Center of Digital Oral and Maxillofacial Defect Repair, Changsha, China; Hunan Provincial Clinical Research Center for Oral Diseases, Changsha, China.
² Department of Stomatology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China; Hunan Provincial Engineering Research Center of Digital Oral and Maxillofacial Defect Repair, Changsha, China; Hunan Provincial Clinical Research Center for Oral Diseases, Changsha, China. Electronic address: fengyunzhi001@csu.edu.cn.
³ Department of Stomatology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China; Hunan Provincial Engineering Research Center of Digital Oral and Maxillofacial Defect Repair, Changsha, China; Hunan Provincial Clinical Research Center for Oral Diseases, Changsha, China. Electronic address: guoyue@csu.edu.cn.

PMID: 38423169
DOI: 10.1016/j.micinf.2024.105316

Abstract

Objects: As periodontitis progresses, the oral microbiome changes dynamically. The aim of this study is to evaluate the dominant bacteria of adults with stage III periodontitis and investigate potential pathways related to the dominant bacteria.

Materials and methods: 16S rRNA sequencing was carried out to detect the differences in the oral microbiome between adult with stage Ⅰ and stage Ⅲ periodontitis and find the dominant bacteria in each group. The inhibitor of the predominant pathway for stage Ⅲ periodontitis was used to investigate the role of the dominant bacteria in periodontitis in vivo and in vitro.

Results: There was no significant difference in the α-diversity between the two groups. The results of β-diversity showed that the samples were divided into different groups according to the stage of periodontitis. The dominant bacteria in youths with stage Ⅲ periodontitis was Prevotella and may be related to the arachidonic acid metabolism pathway. Administration of SKF-86002 suppressed the expression of inflammation mediators in vivo and vitro.

Conclusions: Prevotella was the one dominant bacteria in young people with stage Ⅲ periodontitis and was related to the arachidonic acid metabolism pathway.

Keywords: 16S rRNA sequencing; Arachidonic acid metabolism; Periodontitis; Prevotella.