Tumor characteristics, brain functional activity, and connectivity of tinnitus in patients with vestibular schwannoma: a pilot study

Jiayu Huang; Jiaji Lin; Na You; Xiaolong Li; Yongqin Xiong; Xiaoyu Wang; Haoxuan Lu; Chenxi Li; Runze Li; Jianxing Hu; Jun Zhang; Xin Lou

doi:10.21037/qims-23-721

Tumor characteristics, brain functional activity, and connectivity of tinnitus in patients with vestibular schwannoma: a pilot study

Quant Imaging Med Surg. 2024 Feb 1;14(2):1392-1405. doi: 10.21037/qims-23-721. Epub 2024 Jan 12.

Authors

Jiayu Huang^#¹, Jiaji Lin^#¹, Na You^#², Xiaolong Li^#¹, Yongqin Xiong¹, Xiaoyu Wang¹, Haoxuan Lu¹, Chenxi Li¹, Runze Li¹, Jianxing Hu¹, Jun Zhang², Xin Lou¹

Affiliations

¹ Department of Radiology, Chinese PLA General Hospital/Chinese PLA Medical School, Beijing, China.
² Department of Neurosurgery, Chinese PLA General Hospital/Chinese PLA Medical School, Beijing, China.

^# Contributed equally.

Abstract

Background: The mechanism underlying tinnitus remains unclear, and when it coexists with vestibular schwannoma (VS), it can significantly diminish the quality of life for affected patients. This study aimed to determine the correlation between preoperative clinical characteristics of VS, postoperative changes in brain function, and tinnitus in patients with VS through a cohort study.

Methods: We collected data from 80 patients with VS preoperatively and 28 patients with VS preoperatively and postoperatively, and recruited 28 healthy controls. We used Chi-squared tests and unpaired t-tests to identify clinical characteristics with a significant preoperative effect. We used paired t-tests to identify brain regions where patients demonstrated significant changes in amplitude of low-frequency fluctuation (ALFF) and regional homogeneity (ReHo) postoperatively. Tinnitus severity was evaluated using the Tinnitus Handicap Inventory (THI) and Visual Analogue Scale (VAS). Pearson correlation coefficients were applied to assess the relationship between the changes in ALFF and ReHo and the changes in THI and VAS scores postoperatively. We also conducted seed- and region of interest (ROI)-based functional connectivity (FC) analyses.

Results: Before surgery, patients with VS with tinnitus (n=49) had smaller tumors (t=3.293; P<0.001), more solid tumor (χ²=4.559; P=0.033), and less extrusion into the cerebellum brain stem (χ²=10.345; P=0.001) than those without tinnitus (n=31). After surgery, the 28 patients with VS showed a significant reduction in ALFF in the left Cerebellum_Crus2 (a lobule in the cerebellum anatomy) (ROI 1) and a significant reduction in ReHo in the left Cerebellum_Crus1 (a lobule in the cerebellum anatomy) (ROI 2) and the right precuneus (ROI 3). Conversely, ReHo was significantly increased in the right precentral gyrus (ROI 4) [cluster-level P value family-wise error (P_FWE) <0.05]. The changes in ALFF values were negatively correlated with changes in the VAS score (r=-0.32; P<0.05). The FC strengths of patients between ROI 2 and the left and right posterior cingulate gyrus were significantly decreased postoperatively [false discovery rate (FDR) correction; P<0.05].

Conclusions: Preoperative tinnitus in patients with VS may be influenced by tumor characteristics. The functional activities of brain regions are possibly altered postoperatively, which may be involved in the maintenance of postoperative tinnitus. Notably, the changes in ALFF are correlated with tinnitus.

Keywords: Vestibular schwannoma (VS); functional connectivity (FC); regional homogeneity (ReHo); tinnitus, amplitude of low-frequency fluctuation (ALFF).