Organ-Specific Microsomes from Dark-Grown Hypocotyls of Arabidopsis thaliana

Seinab Noura; Jürgen Kleine-Vehn; Sascha Waidmann

doi:10.1007/978-1-0716-3710-4_29

Organ-Specific Microsomes from Dark-Grown Hypocotyls of Arabidopsis thaliana

Methods Mol Biol. 2024:2772:383-389. doi: 10.1007/978-1-0716-3710-4_29.

Authors

Seinab Noura^{1

2}, Jürgen Kleine-Vehn^{3

4}, Sascha Waidmann³

Affiliations

¹ Institute of Biology II, Chair group of Molecular Plant Physiology (MoPP), University of Freiburg, Freiburg, Germany. seinab.noura@biologie.uni-freiburg.de.
² Center for Integrative Biological Signalling Studies (CIBSS), University of Freiburg, Freiburg, Germany. seinab.noura@biologie.uni-freiburg.de.
³ Institute of Biology II, Chair group of Molecular Plant Physiology (MoPP), University of Freiburg, Freiburg, Germany.
⁴ Center for Integrative Biological Signalling Studies (CIBSS), University of Freiburg, Freiburg, Germany.

PMID: 38411830
DOI: 10.1007/978-1-0716-3710-4_29

Abstract

In this book chapter, we present a method for microsome isolation from the hypocotyl tissue of dark-grown Arabidopsis thaliana. Microsomes are heterogeneous, vesicle-like membranes, which are, not exclusively, derived but enriched with membranes of the endoplasmic reticulum (ER). Here, we describe the experimental setup, including sample preparation, homogenization, differential centrifugation steps, and quality control measures after microsome isolation.

Keywords: Arabidopsis thaliana; Dark-grown hypocotyl; Endoplasmic reticulum (ER); Membrane-bound protein; Microsomes; Ultracentrifugation.

MeSH terms

Arabidopsis*
Centrifugation
Endoplasmic Reticulum
Hypocotyl
Microsomes