Using ER-Targeted Photoconvertible Fluorescent Proteins in Living Plant Cells

Jaideep Mathur; Puja Puspa Ghosh

doi:10.1007/978-1-0716-3710-4_22

Using ER-Targeted Photoconvertible Fluorescent Proteins in Living Plant Cells

Methods Mol Biol. 2024:2772:291-299. doi: 10.1007/978-1-0716-3710-4_22.

Authors

Jaideep Mathur¹, Puja Puspa Ghosh²

Affiliations

¹ Laboratory of Plant Development & Interactions, Department of Molecular & Cellular Biology, University of Guelph, Guelph, ON, Canada. jmathur@uoguelph.ca.
² Laboratory of Plant Development & Interactions, Department of Molecular & Cellular Biology, University of Guelph, Guelph, ON, Canada.

PMID: 38411823
DOI: 10.1007/978-1-0716-3710-4_22

Abstract

Photoconvertible fluorescent proteins (pcFPs) enable differential coloring of a single organelle. Several pcFP-based probes have been targeted to the endoplasmic reticulum (ER) and can serve as useful tools to study ER dynamics and interactions with other organelles. Here, we describe the procedure to conduct live-cell imaging experiments using ER-targeted pcFP-based probes. Potential problems that might occur during the experiments, their solutions, and several ways to improve the experiments are discussed.

Keywords: Dendra; Endoplasmic reticulum; Kaede; Live-cell imaging; Organellar dynamics; Photoconvertible fluorescent proteins; mEos; mMaple.

MeSH terms

Coloring Agents
Endoplasmic Reticulum*
Plant Cells*

Substances

Coloring Agents