Exosomes Derived from Adipose Stem Cells Enhance Angiogenesis in Diabetic Wound Via miR-146a-5p/JAZF1 Axis

Dehui Che; Xinjian Xiang; Juan Xie; Zenghong Chen; Qiong Bao; Dongsheng Cao

doi:10.1007/s12015-024-10685-8

Exosomes Derived from Adipose Stem Cells Enhance Angiogenesis in Diabetic Wound Via miR-146a-5p/JAZF1 Axis

Stem Cell Rev Rep. 2024 Feb 23. doi: 10.1007/s12015-024-10685-8. Online ahead of print.

Authors

Dehui Che^#¹, Xinjian Xiang^#¹, Juan Xie¹, Zenghong Chen¹, Qiong Bao¹, Dongsheng Cao²

Affiliations

¹ Department of Plastic and Reconstructive, The Second Affiliated Hospital of Anhui Medical University, Hefei, China.
² Department of Plastic and Reconstructive, The Second Affiliated Hospital of Anhui Medical University, Hefei, China. aesthetic12345@sina.com.

^# Contributed equally.

PMID: 38393667
DOI: 10.1007/s12015-024-10685-8

Abstract

Chronic trauma in diabetes is a leading cause of disability and mortality. Exosomes show promise in tissue regeneration. This study investigates the role of exosomes derived from adipose stem cells (ADSC-Exos) in angiogenesis. MiRNA-seq analysis revealed significant changes in 47 genes in human umbilical vein endothelial cells (HUVECs) treated with ADSC-Exos, with miR-146a-5p highly expressed. MiR-146a-5p mimics enhanced the pro-angiogenic effects of ADSC-Exos, while inhibitors had the opposite effect. JAZF1 was identified as a direct downstream target of miR-146a-5p through bioinformatics, qRT-PCR, and dual luciferase assay. Overexpress of JAZF1 resulted in decreased proliferation, migration, and angiogenic capacity of HUVECs, and reduced VEGFA expression. This study proposes that ADSC-Exos regulate angiogenesis partly via the miR-146a-5p/JAZF1 axis.

Keywords: Adipose stem cells; Angiogenesis; Diabetic wound; Exosomes; JAZF1; miR-146a-5p.