Prostaglandin pathway activation in the diatom Skeletonema marinoi under grazer pressure

Abstract

Prostaglandins (Pgs) are eicosanoid lipid mediators detected in all vertebrates, in some marine invertebrates, macroalgae and in diatoms, a class of eukaryotic microalgae composing the phytoplankton. The enzymes involved in the Pgs pathway were found to be differentially expressed in two strains of the diatom Skeletonema marinoi, named FE7 and FE60, already known to produce different levels of oxylipins, a class of secondary metabolites involved in the defence of diatoms against copepod predation, with FE7 being higher producer than FE60. In the present study we investigated the response of genes involved in the production of oxylipins and Pgs, evaluating their expression after the exposure to the copepod Temora stylifera. Our results highlighted a grazer feeding preference for FE60, the strain having low oxylipins content and reduced expression of Pgs enzymes, and an impact on the gene expression of the enzymes involved in oxylipins (i.e. lipoxygenase) and Pgs (i.e. cyclooxygenase) biosynthesis, especially in FE7. A time course evaluation of the gene expression over 24 h showed an upregulation of the essential enzyme in the Pgs pathway, the cyclooxygenase, in FE60 after 6 h of exposure to the grazer, differently from FE7 where no upregulation of gene expression in the presence of copepods was revealed. These results provide preliminary indications regarding the existence of a complex involvement of the Pgs pathway in the prey-predator interaction that requires further investigations.