Carbon-negative and high-rate nutrient recovery from municipal wastewater using mixotrophic Scenedesmus acuminatus

Abstract

The efficiency of mixotrophic microalgae in enhancing the recovery of waste nutrients has been well established; however, the recovery rate is crucial in meeting the needs of field applications. This study evaluated the impact of media characteristics on nutrient recovery under mixotrophic conditions. The mixotrophic N recovery rate with S. acuminatus in modified BG-11 reached 2.59 mg L^-1h^-1. A mixotrophic growth optimization strategy was applied to achieve a high-rate nutrient recovery from municipal wastewater treatment plant effluents. The contribution of waste chemical oxygen demand (COD) to nutrient recovery was assessed using secondary effluent (SE) under heterotrophy. The results highlighted a significant increase in total nitrogen (TN) and total phosphorus (TP) recovery rates when glucose was supplied, indicating the additional carbon requirements for efficient nutrient recovery. The TN and TP recovery rates under mixotrophic conditions with the addition of trace metals and high cell density were enhanced by 91.94% and 92.53%, respectively, resulting in recovery rates of 3.43 mg L^-1h^-1 and 0.30 mg L^-1h^-1. The same conditions were used for nutrient recovery from primary effluent (PE), and the results were more satisfactory as the TN and TP recovery rates reached 4.79 and 0.55 mg L^-1h^-1, respectively. Additionally, the study estimated the carbon footprints (C-footprints) and areal footprints of mixotrophy-based nitrogen recovery. The findings revealed carbon footprints and areal footprints of -15.93 ± 4.57 tCO₂e t^-1 N recovery and 0.53 ± 0.19 m³ m^-2d^-1 wastewater, respectively. This high-rate nutrient recovery, achieved under a carbon-negative (C-negative) budget through mixotrophy, presents a novel strategy for efficiently recovering resources from municipal wastewater, thus facilitating resource recycling and ensuring environmental sustainability.

Keywords: C-footprints; Mixotrophy; Municipal wastewater; Nutrient recovery; Scenedesmus acuminatus; Wastewater characteristics.