Combined effects of hypoxia and ammonia-N exposure on the oxygen consumption, glucose metabolism and amino acid metabolism in hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂)

Jie Cao; Jun Mei; Jing Xie

doi:10.1007/s11259-024-10326-y

Combined effects of hypoxia and ammonia-N exposure on the oxygen consumption, glucose metabolism and amino acid metabolism in hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂)

Vet Res Commun. 2024 Feb 19. doi: 10.1007/s11259-024-10326-y. Online ahead of print.

Authors

Jie Cao¹, Jun Mei^{2

3

4

5}, Jing Xie^{6

7

8

9}

Affiliations

¹ College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China.
² College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China. jmei@shou.edu.cn.
³ National Experimental Teaching Demonstration Center for Food Science and Engineering, Shanghai Ocean University, Shanghai, 201306, China. jmei@shou.edu.cn.
⁴ Shanghai Engineering Research Center of Aquatic Product Processing and Preservation, Shanghai, 201306, China. jmei@shou.edu.cn.
⁵ Key Laboratory of Aquatic Products High-Quality Utilization, Storage and Transportation (Co-Construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Shanghai, 201306, China. jmei@shou.edu.cn.
⁶ College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China. jxie@shou.edu.cn.
⁷ National Experimental Teaching Demonstration Center for Food Science and Engineering, Shanghai Ocean University, Shanghai, 201306, China. jxie@shou.edu.cn.
⁸ Shanghai Engineering Research Center of Aquatic Product Processing and Preservation, Shanghai, 201306, China. jxie@shou.edu.cn.
⁹ Key Laboratory of Aquatic Products High-Quality Utilization, Storage and Transportation (Co-Construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Shanghai, 201306, China. jxie@shou.edu.cn.

PMID: 38374273
DOI: 10.1007/s11259-024-10326-y

Abstract

This study evaluated the influence of hypoxia and ammonia-N co-exposure on oxygen consumption, glucose metabolism and amino acid metabolism in hybrid grouper. The results showed that elevated expression of GLUT1, MCT1, PFK, HK and LDH were induced by co-exposure to hypoxia and ammonia. In addition, co-exposure to hypoxia and ammonia reduced the tolerance of hybrid grouper to ammonia-N. Furthermore, ammonia-N exposure caused an increase in oxygen consumption in hybrid grouper. After ammonia-N exposure for 96 h, 10 amino acids contents and activities of AST and ALT elevated in hybrid grouper muscle. The study revealed that combined exposure to hypoxia and ammonia-N significantly increased glucose metabolism, oxygen consumption and amino acid metabolism in hybrid grouper, and presented significant synergistic effects.

Keywords: Amino acids; Ammonia-N; Glucose metabolism; Grouper; Hypoxia.

Abstract

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