Molecular characterization, expression pattern and immunologic function of CD82a in large yellow croaker (Larimichthys crocea)

Yue Liu; Yulin Bai; Sijing Chen; Fei Pu; Yaxian Li; Hongshu Chi; Zaiyu Zheng; Peng Xu; Tao Zhou

doi:10.3389/fimmu.2024.1301877

Molecular characterization, expression pattern and immunologic function of CD82a in large yellow croaker (Larimichthys crocea)

Front Immunol. 2024 Feb 2:15:1301877. doi: 10.3389/fimmu.2024.1301877. eCollection 2024.

Authors

Yue Liu^#¹, Yulin Bai^#¹, Sijing Chen¹, Fei Pu¹, Yaxian Li¹, Hongshu Chi², Zaiyu Zheng², Peng Xu^{1

3}, Tao Zhou^{1

3}

Affiliations

¹ State Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China.
² Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, China.
³ Fujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen, China.

^# Contributed equally.

Abstract

Visceral white spot disease (VWND) caused by Pseudomonas plecoglossicida poses a major threat to the sustainable development of large yellow croaker (Larimichthys crocea) aquaculture. Genome-wide association analysis (GWAS) and RNA-seq research indicated that LcCD82a play an important role in resistance to visceral white spot disease in L. crocea, but the molecular mechanism of LcCD82a response to P. plecoglossicida infection is still unclear. In this study, we cloned and validated the Open Reading Frame (ORF) sequence of LcCD82a and explored the expression profile of LcCD82a in various tissues of L.crocea. In addition, two different transcript variants (LcCD82a-L and LcCD82a-S) of LcCD82a were identified that exhibit alternative splicing patterns after P. plecoglossicida infection, which may be closely related to the immune regulation during pathogenetic process of VWND. In order to explore the function of LcCD82a, we purified the recombinant protein of LcCD82a-L and LcCD82a-S. The bacterial agglutination and apoptosis function analysis showed that LcCD82a may involve in extracellular bacterial recognition, agglutination, and at the same time participate in the process of antigen presentation and induction of cell apoptosis. Collectively, our studies demonstrate that LcCD82a plays a crucial role in regulating apoptosis and antimicrobial immunity.

Keywords: CD82a; Larimichthys crocea; Pseudomonas plecoglossicida; antigen presentation; apoptosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Genome-Wide Association Study
Perciformes* / genetics
Pseudomonas Infections*
RNA-Seq
Recombinant Proteins

Substances

Recombinant Proteins

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This study was supported by the National key research and development program (2022YFD2401002), the National Sciences Fund for Distinguished Young Scholars (32225049), the National Natural Science Foundation of China (U21A20264), the Seed Industry Innovation and Industrialization Project of Fujian Province ( 2021FJSCZY01).