Sorptive tape-spray tandem mass spectrometry using aluminum foil coated with mixed-mode microparticles

Carlos Calero-Cañuelo; Francisco Antonio Casado-Carmona; Rafael Lucena; Soledad Cárdenas

doi:10.1016/j.talanta.2024.125774

Sorptive tape-spray tandem mass spectrometry using aluminum foil coated with mixed-mode microparticles

Talanta. 2024 May 15:272:125774. doi: 10.1016/j.talanta.2024.125774. Epub 2024 Feb 8.

Authors

Carlos Calero-Cañuelo¹, Francisco Antonio Casado-Carmona², Rafael Lucena³, Soledad Cárdenas¹

Affiliations

¹ Affordable and Sustainable Sample Preparation (AS(2)P) Research Group, Departamento de Química Analítica, Instituto Químico para la Energía y el Medioambiente IQUEMA, Universidad de Córdoba, Campus de Rabanales, Edificio Marie Curie, E-14071, Córdoba, Spain.
² Affordable and Sustainable Sample Preparation (AS(2)P) Research Group, Departamento de Química Analítica, Instituto Químico para la Energía y el Medioambiente IQUEMA, Universidad de Córdoba, Campus de Rabanales, Edificio Marie Curie, E-14071, Córdoba, Spain; FI-TRACE Group, Department of Chemistry, Faculty of Science, University of the Balearic Islands, Carretera de Valldemossa Km 7.5, E-07122, Palma de Mallorca, Illes Balears, Spain.
³ Affordable and Sustainable Sample Preparation (AS(2)P) Research Group, Departamento de Química Analítica, Instituto Químico para la Energía y el Medioambiente IQUEMA, Universidad de Córdoba, Campus de Rabanales, Edificio Marie Curie, E-14071, Córdoba, Spain. Electronic address: rafael.lucena@uco.es.

PMID: 38359721
DOI: 10.1016/j.talanta.2024.125774

Abstract

Direct mass spectrometry (MS) is an exciting strategy in bioanalysis, enabling rapid decision-making in different scenarios. Its application is usually hindered by matrix effects and the typically low concentration of the target compounds in the biofluids. In this sense, combining a previous sample preparation step minimizes or removes these shortcomings. This article describes sorptive tape-spray tandem mass spectrometry (STS-MS/MS) based on mixed-mode particles as a strategy to combine sample preparation and MS analysis in a single device. The technique uses a sorptive tape (ST) consisting of mixed-mode polymeric microparticles (combining ionic exchange and hydrophobic interactions) coated over aluminum foil in a spatial controlled way. The tapes act as the sorptive phases to isolate the analytes from the sample matrix and substrates for STS-MS/MS. The performance of the technique has been evaluated by developing a method to determine codeine in saliva as proof of concept. The affordability of the STs elements allows the preparation of many individual phases at low cost so that several samples can be extracted simultaneously, thus increasing the sample throughput. The extraction variables were optimized following a multivariate approach. Working under the optimum conditions, the limit of detection was 0.3 μg L^-1, while the intraday precision, calculated as relative standard deviation (RSD) at three concentration levels, was better than 9.4 %. The accuracy, expressed as relative recovery, was in the range of 78-98 %. The method was also applied to the analysis of real samples. Despite being a powerful strategy, the direct combination of microextraction to MS is not always affordable in all laboratories. For this reason, the STs were also combined with commercial liquid chromatography-MS working under the direct infusion mode to demonstrate the usefulness of the ST in classical extraction workflows.

Keywords: Adhesive tape; Aluminum foil; Ambient ionization mass spectrometry; Codeine; Direct infusion; Mixed-mode sorbent; Saliva samples; Tape-spray.

MeSH terms

Aluminum*
Chromatography, High Pressure Liquid / methods
Tandem Mass Spectrometry* / methods

Substances

Aluminum