Rapid determination of furazolidone residues in animal foods by time-resolved fluorescence immunochromatography

Abstract

Although furazolidone (FZD) was completely banned from livestock production in many countries many years ago due to its mutagenicity and carcinogenicity, the abuse of FZD is still common today. Accurate and rapid detection of FZD residues in animal-derived food products is highly important for human health. Here, a time-resolved fluorescence immunochromatography (TRFI) test strip for rapid and quantitative detection of 3-amino-2-oxazolidinone (AOZ) residues in animal foods was developed and validated. Its limit of detection and limit of quantification were 0.05 and 0.14 μg/kg, respectively. The typical recovery rates were 95-105 % in chicken breast samples spiked with the AOZ standard substance at concentrations of 0.05-2 μg/kg, with a coefficient of variation value ≤8.5 %. The cross-reaction rates of the TRFI-AOZ test strips with 3-amino-5-morpholinomethyl-2-oxazolidone, semicarbazide, and 1-amino-imidazolidin-2,4-dione were less than 1 %. The newly developed TRFI test strip has high sensitivity, high specificity, cost effectiveness and user-friendly control, and is suitable for the rapid and large-scale screening of AOZ residues in animal foods.

Keywords: 3-Amino-2-oxazolidinone (AOZ); Animal foods; Furazolidone (FZD); Rapid and quantitative detection; Time-resolved fluorescence immunochromatography.