Melatonin-Induced Stress Enhanced Biomass and Production of High-Value Secondary Cell Products in Submerged Adventitious Root Cultures of Stevia rebaudiana (Bert.)

Bakht Naz; Aftab Afzal; Hina Ali; Nisar Ahmad; Hina Fazal; Riaz Ullah; Essam A Ali; Mohammad Ali; Zahid Ullah; Ahmad Ali; Shazia Dilbar

doi:10.1021/acsomega.3c07404

Melatonin-Induced Stress Enhanced Biomass and Production of High-Value Secondary Cell Products in Submerged Adventitious Root Cultures of Stevia rebaudiana (Bert.)

ACS Omega. 2024 Jan 26;9(5):5548-5562. doi: 10.1021/acsomega.3c07404. eCollection 2024 Feb 6.

Authors

Bakht Naz¹, Aftab Afzal¹, Hina Ali², Nisar Ahmad³, Hina Fazal⁴, Riaz Ullah⁵, Essam A Ali⁶, Mohammad Ali³, Zahid Ullah⁷, Ahmad Ali⁷, Shazia Dilbar⁷

Affiliations

¹ Department of Botany, Hazara University, Mansehra 21430, Pakistan.
² Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs, School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
³ Center for Biotechnology and Microbiology, University of Swat, Swat 19200, Pakistan.
⁴ Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Peshawar 25120, Pakistan.
⁵ Department of Pharmacognosy College of Pharmacy King Saud University, Riyadh 11451, Saudi Arabi.
⁶ Department of Pharmaceutical Chemistry, College of Pharmacy King Saud University, Riyadh 11451, Saudi Arabia.
⁷ Center for Plant Sciences and Biodiversity, University of Swat, Charbagh Swat 19200, Pakistan.

Abstract

Stress is one of the important factors that directly or indirectly affects the plant architecture, biochemical pathways, and growth and development. Melatonin (MEL) is an important stress hormone; however, the exogenous addition of melatonin to culture media stimulates the defense mechanism and releases higher quantities of secondary metabolites. In this study, submerged adventitious root cultures (SARCs) of diabetically important Stevia rebaudiana were exposed to variable concentrations (0.5-5.0 mg/L) of MEL in combination with 0.5 mg/L naphthalene acetic acid (NAA) to investigate the biomass accumulation during growth kinetics with 07 days intervals for a period of 56 days. The effects of exogenous MEL on the biosynthesis of stevioside (Stev.), total phenolics content (TPC), total flavonoids content (TFC), total phenolics production (TPP), total flavonoids production (TFP), total polyphenolics content (TPPC), fresh and dry weight (FW & DW), and antioxidant potential were also studied. Most of the SARCs displayed lag, exponential, stationary, and decline phases with variable biomass accumulation. The maximum fresh (236.54 g/L) and dry biomass (28.64 g/L) was observed in SARCs exposed to 3.0 mg/L MEL and 0.5 mg/L NAA. The same combination of MEL and NAA also enhanced the accumulation of TPC (18.96 mg/g-DW), TFC (6.33 mg/g-DW), TPP (271.51 mg/L), TFP (90.64 mg/L), and TPPC (25.29 mg/g-DW). Similarly, the highest stevioside biosynthesis (91.45 mg/g-DW) and antioxidant potential (86.15%) were observed in SARCs exposed to 3.0 mg/L MEL and NAA. Moreover, a strong correlation was observed between the biomass and the contents of phenolics, flavonoids, antioxidants, and stevioside. These results suggest that MEL is one of multidimensional stress hormones that modulate the biosynthetic pathways to release higher quantities of metabolites of interest for various industrial applications.