Reference gene selection for quantitative real-time PCR analysis of Hymenopellis radicata under abiotic stress

Luping Cao; Qin Zhang; Renyun Miao; Xu Zhao; Yanqing Ni; Wensheng Li; Rencai Feng; Delong Yang

doi:10.1016/j.funbio.2023.11.004

Reference gene selection for quantitative real-time PCR analysis of Hymenopellis radicata under abiotic stress

Fungal Biol. 2024 Feb;128(1):1567-1577. doi: 10.1016/j.funbio.2023.11.004. Epub 2023 Nov 24.

Authors

Luping Cao¹, Qin Zhang², Renyun Miao², Xu Zhao³, Yanqing Ni⁴, Wensheng Li⁴, Rencai Feng², Delong Yang⁵

Affiliations

¹ College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, Gansu, China.
² Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610299, Sichuan, China; Chengdu National Agricultural Science and Technology Center, Chengdu 610299, Sichuan, China.
³ Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610299, Sichuan, China; Chengdu National Agricultural Science and Technology Center, Chengdu 610299, Sichuan, China. Electronic address: zhaoxu02@caas.cn.
⁴ College of Food and Biological Engineering, Chengdu University, Chengdu 610106, Sichuan, China.
⁵ College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, Gansu, China. Electronic address: yangdl@gsau.edu.cn.

PMID: 38341262
DOI: 10.1016/j.funbio.2023.11.004

Abstract

Hymenopellis radicata (H. radicata) is an edible fungus rich in protein and mineral elements, with high edible and medical value. And reference genes suitable for normalization of qRT-PCR data from this species have not been investigated. In this study, therefore, we selected 11 housekeeping genes common in biology. The expression levels of these housekeeping genes were measured in three different tissues and six different abiotic stress treatments in mycelium. They were evaluated for expression stability using online tools. The results showed that gene ACT could be stable expressed in all samples. The expressions of genes TUB and UBQ10 are the most stable under heat stress, ACT and EF are the most stable genes under salt stress, ACT and TUB are the most stable genes under oxidation stress, RPL6 and EF are the most stable genes under pH condition, ACT and RPB2 are the most stable genes under cadmium stress, and RPB2 and UBC are the most stable genes under drought condition. ACT and PP2A are the most stable genes at different tissue sites. This study is of great help to explore the gene expression pattern of H. radicata, and also provides reference for internal reference gene screening under other conditions.

Keywords: Abiotic stresses; Hymenopellis radicata; Normalization; qRT-PCR; reference gene.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agaricales*
Gene Expression Profiling
Gene Expression Regulation, Plant*
Genes, Plant*
Real-Time Polymerase Chain Reaction / methods
Stress, Physiological / genetics

Supplementary concepts

Hymenopellis radicata