Understanding the occurrence and transformation of microplastics when released into the environment is essential for risk assessment. The use of biodegradable polymers in agriculture can help to reduce microplastic accumulation in soil, since released fragments of such materials are not persistent and are further transformed into CO2 and biomass (Wohlleben et al., 2023). To be able to monitor the fragmentation and biodegradation of these materials in soil, a validated extraction protocol is needed, which does not induce changes in the chemical and particle properties, additionally it should show high recoveries and matrix removal efficiency. A density-based extraction method in the centrifuge has the potential to remove a high amount of the soil matrix and is very selective for the polymer at the same time. Here we developed an efficient and non-destructive extraction protocol for biodegradable fragments from different soils using sequential centrifugation steps with varying densities and a freezing approach for sample collection. Although the focus of the present study was on biodegradable fragments, the technique can also be used for other types of microplastics with similar or lower density than the one tested for the method validation, but additional recovery tests for the target analyte are recommended.•A density-based extraction method for microplastics from soil, validated by recovery and stability tests using biodegradable polymers•Vessel changes and harsh chemical treatments are kept to a minimum.
Keywords: Centrifugation; Centrifugation-based microplastic extraction technique from soil; Complex matrices; Density separation; Environment; Extraction; Microplastics; Soil.
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