Here, spherical Mn2O3 nanozymes were synthesized via a one-step green method using different green precursors, and their physicochemical properties and biological activities were monitored with various green precursors. Powder X-ray diffraction (PXRD) was performed to determine the crystalline properties and phases involved in the formation of cubic Mn2O3 nanozymes. The synthesized nanozymes were spherical and examined by SEM and FESEM studies. All of the samples synthesized using different green precursors exhibited different sizes but similar spherical shapes. Moreover, all green-synthesized nanozymes catalyzed the oxidation reaction of the chromogenic substrate 3,3'5,5' tetramethylbenzidine (TMB) in the absence of H2O2, and A2 (lemon-mediated Mn2O3 nanozymes), which the followed Michaelis-Menten kinetics, showed the best activity. Therefore, A2 (lemon-mediated nanozyme) showed oxidase-mimicking activity with distinct Km and Vmax values calculated by the Lineweaver-Burk plot. Furthermore, the current nanozymes demonstrated a significant ability to kill both Gram-negative and Gram-positive bacteria as well as effectively destroy biofilms under physiological conditions. Moreover, the green-mediated nanozymes also displayed ROS-scavenging activity. Our nanozymes exhibited scavenging activity toward OH and O2-• radicals and metal chelation activity, which were investigated colorimetrically. Therefore, these nanozymes might be used as effective antibacterial agents and also for the consumption of reactive oxygen species.
Keywords: antibacterial activity; antioxidant activity; coprecipitation; oxidase at pH 5; plant extract.